Explore topic-wise MCQs in Biochemistry.

This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.

1.

A purine with an amine (NH) group on the 6 carbon is

A. adenine
B. cytosine
C. thymine
D. guanine
Answer» B. cytosine
2.

Because DNA is a highly charged polyanion, its stability to heat denaturation

A. does not depend on hydrophobic interactions
B. increases with increasing salt
C. is independent of G - C content
D. decreases with increasing salt
Answer» C. is independent of G - C content
3.

The sugar in RNA is __________ , the sugar in DNA is __________

A. deoxyribose, ribose
B. ribose, deoxyribose
C. ribose, phosphate
D. ribose, uracil
Answer» C. ribose, phosphate
4.

Nucleoside is a pyrimidine or purine base

A. covalently bonded to a sugar
B. ionically bonded to a sugar
C. hydrogen bonded to a sugar
D. none of the above
Answer» B. ionically bonded to a sugar
5.

In gel electrophoresis, what fragments will move most quickly through a gel?

A. Large fragments
B. Small fragments
C. Large genome
D. None of these
Answer» C. Large genome
6.

Which pyrimidine base contains an amino group at carbon 4?

A. Cytosine
B. Thymine
C. Uracil
D. Adenine
Answer» B. Thymine
7.

The unfavourable free energy associated with not satisfying a hydrogen bond after formation of the double helix is approximately

A. 10 kJ/mol
B. 20 kJ/mol
C. 25 kJ/mol
D. 30 kJ/mol
Answer» C. 25 kJ/mol
8.

The most stabilizing force for nucleic acids is

A. hydrogen bonds
B. electrostatic bond
C. Van der Waals
D. conformational entropy
Answer» D. conformational entropy
9.

A purine with an amine (NH2) group on the 6th carbon is

A. adenine
B. cytosine
C. thymine
D. guanine
Answer» B. cytosine
10.

Which of the following is found on RNA but not DNA?

A. Uracil
B. Deoxyribose
C. Phosphate
D. Adenine
Answer» B. Deoxyribose
11.

Nucleotide bases and aromatic amino acids absorb light respectively at

A. 280 and 260 nm
B. 260 and 280 nm
C. 270 and 280 nm
D. 260 and 270 nm
Answer» C. 270 and 280 nm
12.

Nucleic acids can be analyzed experimentally by their

A. molecular weight
B. absorption of visible light
C. absorption of uv light
D. none of these
Answer» D. none of these
13.

Thymidine

A. can participate in hydrophobic interactions due to its methyl group
B. is replaced by uracil in RNA
C. normally forms two hydrogen bonds with adenosine
D. all of the above
Answer» E.
14.

A nucleotide consists of

A. a sugar, a base and a phosphate
B. a sugar and a phosphate
C. paired bases
D. a sugar, a base and three phosphates
Answer» B. a sugar and a phosphate
15.

Which of the following is a purine?

A. Cytosine
B. Adenine
C. Thymine
D. Uracil
Answer» C. Thymine
16.

A five carbon sugar lacking a hydrogen at the number 2 carbon is found in

A. DNA
B. RNA
C. mRNA
D. rDNA
Answer» B. RNA