Explore topic-wise MCQs in Microbiology.

This section includes 229 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.

101.

The organisms retained in the fluids filtered by Seitz filter is

A. Proteus
B. Staphylococcus
C. Clostridium
D. None of these
Answer» E.
102.

The effectiveness of heat in killing microorganisms is much greater in _____________

A. acid
B. alkali
C. neutral
D. acid and alkali
Answer» B. alkali
103.

Gram-negative organisms are more susceptible to higher concentrations of triphenylmethane dyes.

A. True
B. False
C. May be
D. Can't say
Answer» B. False
104.

The endosymbiosis hypothesis provides an explanation for how

A. eukaryotes developed from prokayotes
B. prokaryotes developed from eukaryotes
C. algae developed from protozoa
D. protozoa developed from algae
Answer» B. prokaryotes developed from eukaryotes
105.

Which bacteria appears purple-violet colour after staining?

A. Gram-positive
B. Gram-negative
C. Both Gram-positive and Gram-negative
D. Neither Gram-positive nor Gram-negative
Answer» B. Gram-negative
106.

Gram-positive bacteria are usually more susceptible to?

A. streptomycin
B. tetracyclin
C. penicillin
D. ampicillin
Answer» D. ampicillin
107.

The dye eosinate of methylene blue belongs to which group?

A. Acidic dye
B. Basic dye
C. Neutral dye
D. Oxazine dye
Answer» D. Oxazine dye
108.

Which of the following is the example of Gram-negative bacteria?

A. Lactobacillus
B. Eschericia coli
C. Staphylococcus aureus
D. Bacillus subtilis
Answer» C. Staphylococcus aureus
109.

Which of the staining technique helps in demonstrating spore structure in bacteria as well as free spores?

A. Acid-fast stain
B. Endospore stain
C. Capsule stain
D. Flagella stain
Answer» C. Capsule stain
110.

The DNA of Klebsiella pneumonia has a moles % G+C content of?

A. 70-71
B. 50-53
C. 56-58
D. 32-35
Answer» D. 32-35
111.

To transfer genes from one species to another, especially useful are

A. vectors only
B. plasmids only
C. double stranded DNA molecules
D. vectors and plasmids
Answer» E.
112.

Micro-organisms do not include

A. mushrooms
B. molds
C. bacteria
D. fungi
Answer» D. fungi
113.

Which of the following are characteristics of archaebacterial different from eubacteria?

A. methane-producers
B. extreme halophiles
C. thermoacidophiles
D. methane-producers,extreme halophiles,and thermoacidophiles
Answer» E.
114.

What are the blood serum proteins produced by animals called?

A. Enzymes
B. Antibodies
C. Amino acids
D. Toxins
Answer» C. Amino acids
115.

The DNA molecule of microorganisms is made up of base pairs of ________________

A. guanine-cytosine
B. adenine-thymine
C. adenine-cytosine
D. guanine-cytosine and adenine-thymine
Answer» E.
116.

According to Bergey’s Manual of Systematic Bacteriology, prokaryotes that lack a cell wall belong to the group?

A. Gracilicutes
B. Firmicutes
C. Tenericutes
D. Mendosicutes
Answer» D. Mendosicutes
117.

If two organisms are distantly related and show no DNA homology there still may be similarity in their rRNA cistrons.

A. True
B. False
C. May be
D. Can't say
Answer» B. False
118.

Which of the following actions are not affected by antimicrobial agents?

A. cell wall synthesis
B. nucleic acid synthesis
C. protein synthesis
D. capsule formation
Answer» E.
119.

A(n)____is used to prevent infection by killing or inhibiting pathogen growth on animal tissues.

A. bacteriostatic agent
B. sanitizer
C. disinfectant
D. antiseptic
Answer» E.
120.

Which among the following come under Gram-positive eubacteria?

A. Clostridium
B. Actinomyces
C. Rhizobium
D. Clostridium,Actinomyces
Answer» E.
121.

Which of the following agents irreversibly oxidize and inactivate proteins with sulphydral groups?

A. Alcohol
B. Phenol
C. Iodine
D. Chlorine
Answer» D. Chlorine
122.

Gonococci are inhibited by tryptoflavine in dilutions of ____________________

A. 1:200,000
B. 1:300,000
C. 1:1,000,000
D. 1:10,000,000
Answer» E.
123.

Chloramines are more stable than than hypochlorites.

A. True
B. False
C. May be
D. Can't say
Answer» B. False
124.

Which of the following structure is absent in eukaryotic cells?

A. Mitochondria
B. Chloroplasts
C. Golgi structure
D. Mesosome
Answer» E.
125.

Steam exposure of a material at 100°C for 20 minutes for three consecutive days is known as

A. inspissation
B. tyndallization
C. autoclaving
D. none of these
Answer» C. autoclaving
126.

Which of the following sequences has helped in identifying eukaryotes, eubacteria and archeabacterial cell types?

A. Signature sequence
B. Signal sequence
C. Shine-Dalgarno sequence
D. Amino acid sequence
Answer» B. Signal sequence
127.

Which of the following is the most primitive?

A. Virus
B. Eukaryote
C. Archaeon
D. Mitochondria
Answer» D. Mitochondria
128.

Two organisms which are very closely related to each other have which of the following property?

A. similar mol% G+C values
B. different mol% G+C values
C. similar mol% G+C values and heteroduplexes are formed.
D. different mol% G+C values and heteroduplexes are not formed.
Answer» D. different mol% G+C values and heteroduplexes are not formed.
129.

All the following are considered eukaryotes except

A. archaea
B. fungi
C. protozoa
D. humans
Answer» B. fungi
130.

What are ribosomes composed of?

A. Proteins
B. DNA
C. RNA
D. Proteins and RNA
Answer» E.
131.

Type strain is used for referring to?

A. species
B. genus
C. family
D. division
Answer» B. genus
132.

The correct order of taxonomic groups from higher to lower rank is?

A. Kingdom—Order—Class—Family
B. Order—Class—Division—Family—Genus—Species
C. Kingdom—Order—Division—Family—Class—Genus—Species
D. Kingdom—Division—Class—Order—Family—Genus—Species
Answer» E.
133.

Iodine kills the microorganisms by causing denaturation of proteins.

A. True
B. False
C. May be
D. Can't say
Answer» C. May be
134.

Which of the following is the mode of action of formaldehyde?

A. interference with glycolysis
B. shows oxidizing property
C. combines with vital nitrogen compounds
D. changes the permeability of cytoplasmic membrane
Answer» D. changes the permeability of cytoplasmic membrane
135.

Which of the following is a limitation of the autoclave?

A. It takes too long to sterilize
B. It lacks the ability to inactivate viruses
C. It lacks the ability to kill endospores
D. It will destroy heat labile materials
Answer» E.
136.

The lowest temperature that kills all microorganisms in a liquid suspension in 10 minutes is known as the

A. decimal reduction time
B. thermal death point
C. thermal death temperature
D. thermal death time
Answer» C. thermal death temperature
137.

A suspension of E.coli exposed to a heat treatment may yield a greater number of survivors if a plating medium of _____________ is used.

A. peptone
B. trypticase soy agar
C. deoxycholate agar
D. potato dextrose
Answer» C. deoxycholate agar
138.

Which of the following tests is designed to simulate the natural conditions under which the disinfectants are used in hospitals?

A. Phenol coefficient test
B. Minimum inhibitory concentration test
C. Capacity test
D. None of these
Answer» D. None of these
139.

For sterilization of which material gamma rays can be used?

A. Catheters
B. Plastic syringes
C. Canulas
D. None of these
Answer» E.
140.

In Gram-staining, iodine is used as a______________

A. fixative
B. mordant
C. solublizer
D. stain
Answer» C. solublizer
141.

Media containing spores and thermolabile constituents are sterilized by

A. pasteurization
B. UV radiation
C. dry heat
D. tyndalization
Answer» C. dry heat
142.

Which of the following items could be sterilized by dry heat sterilization?

A. Intravenous (IV) solution
B. Plastic IV bags
C. Glass pipettes
D. rubber gloves
Answer» D. rubber gloves
143.

Liquid water-soluble substances appropriately diluted are dispensed into sterile test tubes, to which are added a measured amount of the test organism and then this is transferred into tubes of sterile media.This method helps in determining _____________________

A. whether the inhibitory action is bactericidal
B. whether the inhibitory action is bacteristatic
C. decrease in the amount of growth
D. complete absence of growth
Answer» B. whether the inhibitory action is bacteristatic
144.

Which is the most important surface active disinfectants?

A. Amphoteric compounds
B. Cationic compounds
C. Non-ionic compounds
D. Anionic compounds
Answer» C. Non-ionic compounds
145.

An agent that prevents the growth of bacteria are known as __________________

A. Bactericide
B. Bacteriostatic
C. Antimicrobial
D. Antibiotic
Answer» C. Antimicrobial
146.

Gram staining was introduced by

A. Christian gram
B. Alfred Gram
C. Robertcook
D. Louis Pasteur
Answer» B. Alfred Gram
147.

All of the following are sporicidal except

A. glutaraldehyde
B. ethylene oxide
C. formaldehyde
D. alcohol
Answer» E.
148.

The foundation for the germ theory of disease was set down by

A. Robert Koch
B. Ronald Ross
C. Louis Pasteur
D. Walter Reed
Answer» D. Walter Reed
149.

Which of the following is considered the most unifying concept in biology?

A. Taxonomy
B. Anatomy
C. Genetics
D. Evolution
Answer» E.
150.

Total Magnification is obtained by__________

A. Magnifying power of the objective lens
B. Magnifying power of eyepiece
C. Magnifying power of condenser lens
D. Magnifying power of both the objective lens and eyepiece
Answer» E.