MCQOPTIONS
Saved Bookmarks
This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The process by which every type of transformant can be identified is __________ |
| A. | Replica plating |
| B. | Hybridization |
| C. | Blotting |
| D. | Insertional inactivation |
| Answer» E. | |
| 2. |
Under the influence of which ion does E. coli takes up plasmid from the environment? |
| A. | Nickel |
| B. | Copper |
| C. | Lead |
| D. | Sulphur |
| Answer» C. Lead | |
| 3. |
The vector and the DNA insert are cut by different enzymes for convenience. |
| A. | True |
| B. | False |
| Answer» C. | |
| 4. |
DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
UNDER_THE_INFLUENCE_OF_WHICH_ION_DOES_E._COLI_TAKES_UP_PLASMID_FROM_THE_ENVIRONMENT??$ |
| A. | Nickel |
| B. | Copper |
| C. | Lead |
| D. | Sulphur |
| Answer» C. Lead | |
| 6. |
The_process_by_which_every_type_of_transformant_can_be_identified_is___________$ |
| A. | Replica plating |
| B. | Hybridization |
| C. | Blotting |
| D. | Insertional inactivation |
| Answer» E. | |
| 7. |
Which of the following is the primary use of an expression vector? |
| A. | DNA library |
| B. | DNA purification |
| C. | Protein production |
| D. | DNA cloning |
| Answer» D. DNA cloning | |
| 8. |
What is the major difference between cloning vectors and primary vectors? |
| A. | Selectable marker |
| B. | DNA inserts |
| C. | Presence of promoter |
| D. | Presence of two Ori |
| Answer» D. Presence of two Ori | |
| 9. |
The vector and the DNA insert are cut by different enzymes for convenience __________ |
| A. | True |
| B. | False |
| Answer» C. | |
| 10. |
DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme _________ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 11. |
Which of the following is an essential feature for being a perfect vector? |
| A. | Origin of replication |
| B. | Selectable marker |
| C. | Restriction site |
| D. | Virulent gene |
| Answer» E. | |
| 12. |
Which is the most common organism considered for genetic manipulations? |
| A. | E. coli |
| B. | Saccharomyces cerevisiae |
| C. | Cyanobacteria |
| D. | Bacillus Subtilis |
| Answer» B. Saccharomyces cerevisiae | |
| 13. |
Transformation does not involve ____________ |
| A. | Cutting |
| B. | Recombination |
| C. | Propagation |
| D. | Expression |
| Answer» E. | |
| 14. |
During DNA cloning which of the following is not a crucial requirement? |
| A. | DNA inserts |
| B. | Vector |
| C. | Protein expression |
| D. | Molecular cutter |
| Answer» D. Molecular cutter | |