 
			 
			MCQOPTIONS
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				This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | The process by which every type of transformant can be identified is __________ | 
| A. | Replica plating | 
| B. | Hybridization | 
| C. | Blotting | 
| D. | Insertional inactivation | 
| Answer» E. | |
| 2. | Under the influence of which ion does E. coli takes up plasmid from the environment? | 
| A. | Nickel | 
| B. | Copper | 
| C. | Lead | 
| D. | Sulphur | 
| Answer» C. Lead | |
| 3. | The vector and the DNA insert are cut by different enzymes for convenience. | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 4. | DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme. | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 5. | UNDER_THE_INFLUENCE_OF_WHICH_ION_DOES_E._COLI_TAKES_UP_PLASMID_FROM_THE_ENVIRONMENT??$ | 
| A. | Nickel | 
| B. | Copper | 
| C. | Lead | 
| D. | Sulphur | 
| Answer» C. Lead | |
| 6. | The_process_by_which_every_type_of_transformant_can_be_identified_is___________$ | 
| A. | Replica plating | 
| B. | Hybridization | 
| C. | Blotting | 
| D. | Insertional inactivation | 
| Answer» E. | |
| 7. | Which of the following is the primary use of an expression vector? | 
| A. | DNA library | 
| B. | DNA purification | 
| C. | Protein production | 
| D. | DNA cloning | 
| Answer» D. DNA cloning | |
| 8. | What is the major difference between cloning vectors and primary vectors? | 
| A. | Selectable marker | 
| B. | DNA inserts | 
| C. | Presence of promoter | 
| D. | Presence of two Ori | 
| Answer» D. Presence of two Ori | |
| 9. | The vector and the DNA insert are cut by different enzymes for convenience __________ | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 10. | DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme _________ | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 11. | Which of the following is an essential feature for being a perfect vector? | 
| A. | Origin of replication | 
| B. | Selectable marker | 
| C. | Restriction site | 
| D. | Virulent gene | 
| Answer» E. | |
| 12. | Which is the most common organism considered for genetic manipulations? | 
| A. | E. coli | 
| B. | Saccharomyces cerevisiae | 
| C. | Cyanobacteria | 
| D. | Bacillus Subtilis | 
| Answer» B. Saccharomyces cerevisiae | |
| 13. | Transformation does not involve ____________ | 
| A. | Cutting | 
| B. | Recombination | 
| C. | Propagation | 
| D. | Expression | 
| Answer» E. | |
| 14. | During DNA cloning which of the following is not a crucial requirement? | 
| A. | DNA inserts | 
| B. | Vector | 
| C. | Protein expression | 
| D. | Molecular cutter | 
| Answer» D. Molecular cutter | |