MCQOPTIONS
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MCQOPTIONS
This section includes 29 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which part of the light microscope controls the intensity of light entering the viewing area? |
| A. | Coarse adjustment screw |
| B. | Fine adjustment screw |
| C. | Diaphragm |
| D. | Condenser lens |
| Answer» D. Condenser lens | |
| 2. |
In Phase contrast microscopy, the rate at which light enters through objects is __________ |
| A. | Constant |
| B. | Inversely proportional to their refractive indices |
| C. | Directly proportional to their refractive indices |
| D. | Exponentially related to their refractive indices |
| Answer» C. Directly proportional to their refractive indices | |
| 3. |
Total Magnification is obtained by __________ |
| A. | Magnifying power of the objective lens |
| B. | Magnifying power of eyepiece |
| C. | Magnifying power of condenser lens |
| D. | Magnifying power of both the objective lens and eyepiece |
| Answer» E. | |
| 4. |
Resolving power of a microscope is a function of ____________ |
| A. | Wavelength of light used |
| B. | Numerical aperture of lens system |
| C. | Refractive index |
| D. | Wavelength of light used and numerical aperture of lens system |
| Answer» E. | |
| 5. |
Rhodamine is a ___________________ |
| A. | fluorochrome |
| B. | antibody |
| C. | stain |
| D. | gel type |
| Answer» B. antibody | |
| 6. |
Which of the following gives a three dimensional quality of the image? |
| A. | Differential interference contrast |
| B. | Bright-field microscope |
| C. | Gas chromatography |
| D. | Spectrophotometry |
| Answer» B. Bright-field microscope | |
| 7. |
Unstained specimens can be visualized using ________________ |
| A. | bright-field microscope |
| B. | phase-contrast microscope |
| C. | mass spectroscopy |
| D. | spectrophotometer |
| Answer» C. mass spectroscopy | |
| 8. |
Slides containing adherent paraffin sections are immersed in __________________ |
| A. | toluene |
| B. | isoamyl alcohol |
| C. | isopropanol |
| D. | acetic acid |
| Answer» B. isoamyl alcohol | |
| 9. |
Which of the following can not be used as a fixative? |
| A. | formaldehyde |
| B. | acetic acid |
| C. | alcohol |
| D. | vitamin B12 |
| Answer» E. | |
| 10. |
A fixative _______________ the macromolecular components of a cell. |
| A. | degrades |
| B. | immobilizes |
| C. | penetrates |
| D. | granulates |
| Answer» C. penetrates | |
| 11. |
Which of the following is used for the preparation of a section specimen? |
| A. | phenol |
| B. | isoamyl alcohol |
| C. | fixative |
| D. | lubricative |
| Answer» D. lubricative | |
| 12. |
The Feulgen stain is used for staining ____________________ |
| A. | plasma membrane |
| B. | chromosomes |
| C. | phospholipids |
| D. | mitochondria |
| Answer» C. phospholipids | |
| 13. |
The maximum possible numerical aperture of 1.5 is for a ______________ lens. |
| A. | oil immersion |
| B. | air interface |
| C. | water interface |
| D. | binocular |
| Answer» B. air interface | |
| 14. |
The numerical aperture is constant for each lens. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 15. |
527 nanometers is the wavelength of ______________ light. |
| A. | red |
| B. | white |
| C. | green |
| D. | blue |
| Answer» D. blue | |
| 16. |
The resolution attained by a microscope is limited by _____________________ |
| A. | diffraction |
| B. | refraction |
| C. | reflection |
| D. | retraction |
| Answer» B. refraction | |
| 17. |
Ocular lens forms a virtual image. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 18. |
Which of the following magnification of the ocular will occupy maximum retinal surface? |
| A. | 1X |
| B. | 5X |
| C. | 20X |
| D. | 50X |
| Answer» E. | |
| 19. |
Which of the following component of the light microscope illuminates the specimen by gathering diffuse rays from the light microscope? |
| A. | light source |
| B. | eyepiece |
| C. | condenser lens |
| D. | screws |
| Answer» D. screws | |
| 20. |
IN_PHASE_CONTRAST_MICROSCOPY,_THE_RATE_AT_WHICH_LIGHT_ENTERS_THROUGH_OBJECTS_IS__________?$ |
| A. | Constant |
| B. | Inversely proportional to their refractive indices |
| C. | Directly proportional to their refractive indices |
| D. | Exponentially related to their refractive indices |
| Answer» C. Directly proportional to their refractive indices | |
| 21. |
Which_part_of_the_light_microscope_controls_the_intensity_of_light_entering_the_viewing_area?$ |
| A. | Coarse adjustment screw |
| B. | Fine adjustment screw |
| C. | Diaphragm |
| D. | Condenser lens |
| Answer» D. Condenser lens | |
| 22. |
In light microscopy, which of the following is used as fixatives prior to staining technique? |
| A. | Osmic acid |
| B. | Glutaraldehyde |
| C. | Heat |
| D. | Osmic acid, glutaraldehyde, heat |
| Answer» D. Osmic acid, glutaraldehyde, heat | |
| 23. |
Total Magnification is obtained by__________ |
| A. | Magnifying power of the objective lens |
| B. | Magnifying power of eyepiece |
| C. | Magnifying power of condenser lens |
| D. | Magnifying power of both the objective lens and eyepiece |
| Answer» E. | |
| 24. |
In fluorescence microscopy, which of the following performs the function of removing all light except the blue light? |
| A. | Exciter filter |
| B. | Barrier filter |
| C. | Dichroic mirror |
| D. | Mercury arc lamp |
| Answer» B. Barrier filter | |
| 25. |
Oil immersion objective lens has an NA value of____________ |
| A. | 0.65 |
| B. | 0.85 |
| C. | 1.33 |
| D. | 1.00 |
| Answer» D. 1.00 | |
| 26. |
The greatest resolution in light microscopy can be obtained with ___________ |
| A. | Longest wavelength of visible light used |
| B. | An objective with minimum numerical aperture |
| C. | Shortest wavelength of visible light used |
| D. | Shortest wavelength of visible light used and an objective with the maximum numerical aperture |
| Answer» E. | |
| 27. |
Resolving power of a microscope is a function of____________ |
| A. | Wavelength of light used |
| B. | Numerical aperture of lens system |
| C. | Refractive index |
| D. | Wavelength of light used and numerical aperture of lens system |
| Answer» E. | |
| 28. |
What is the minimum distance for the eye to focus any object? |
| A. | 11 cm |
| B. | 25 cm |
| C. | 32 cm |
| D. | 4 2 cm |
| Answer» C. 32 cm | |
| 29. |
Which part of the compound microscope helps in gathering and focusing light rays on the specimen to be viewed? |
| A. | Eyepiece lens |
| B. | Objective lens |
| C. | Condenser lens |
| D. | Magnifying lens |
| Answer» D. Magnifying lens | |