MCQOPTIONS
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MCQOPTIONS
This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Enzyme Technology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
An enzyme with a Km of 5mM has a reaction rate of 100 mmol/min at substrate concentration of 0.25 mmol. What is the maximum reaction rate that this enzyme can achieve when its saturated with substrate? |
| A. | 2100 |
| B. | 1500 |
| C. | 1900 |
| D. | 9000 |
| Answer» B. 1500 | |
| 2. |
Given an enzyme with Km of 0.25mM, at what substrate concentration will the velocity of the enzyme reach 1/2 of the Vmax? (Vmax = 100 mmol/s) |
| A. | 0.5 mM |
| B. | 0.25 mM |
| C. | 0.75 mM |
| D. | 25 mM |
| Answer» C. 0.75 mM | |
| 3. |
A low Km value points out a low affinity of an enzyme towards its substrate. |
| A. | True |
| B. | False |
| Answer» C. | |
| 4. |
______________ is defined as an enzyme molecule s efficiency to convert maximum substrate molecules into product per unit time. |
| A. | Catalytic efficiency |
| B. | Miachelis Menten constant |
| C. | Turn over number |
| D. | V<sub>max</sub> |
| Answer» D. V<sub>max</sub> | |
| 5. |
The equation which is based on reciprocal form of Miachelis Menten equation at constant [Et] level is referred to as ______________ |
| A. | Eadie-Hofstee plot equation |
| B. | Eisenthal and Cornish and Bowden plot equation |
| C. | Lineweaver Burk equation |
| D. | Hanes plot equation |
| Answer» C. Lineweaver Burk equation | |
| 6. |
Which of the following equation is Eadie-Hofstee plot equation? |
| A. | ( frac{1}{V_0} = frac{K_m}{V_{max}} frac{1}{[S]}+ frac{1}{V_{max}} ) |
| B. | (V_0= frac{V_{max} [S]}{K_m+[S]} ) |
| C. | (V_0=K_m frac{V_0}{S_0}+V_{max} ) |
| D. | ( frac{[S_0]}{V_0} = frac{1}{V_{max}} [S_0]+ frac{K_m}{V_{max}} ) |
| Answer» D. ( frac{[S_0]}{V_0} = frac{1}{V_{max}} [S_0]+ frac{K_m}{V_{max}} ) | |
| 7. |
Which of the following is termed as catalytic efficiency? |
| A. | K<sub>cat</sub> |
| B. | K<sub>m</sub> |
| C. | K<sub>cat</sub>/K<sub>m</sub> |
| D. | V<sub>max</sub> |
| Answer» D. V<sub>max</sub> | |
| 8. |
What does the equation (V_0= frac{V_{max} [S]}{K_m+[S]} ) represent? |
| A. | Lineweaver Burk equation |
| B. | Miachelis Menten equation |
| C. | Eadie-Hofstee plot equation |
| D. | Hanes plot equation |
| Answer» C. Eadie-Hofstee plot equation | |