MCQOPTIONS
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MCQOPTIONS
This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Technology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following is the best method for isolating enzymes from cell free extract? |
| A. | pH treatment |
| B. | Temperature treatment |
| C. | Chemical treatment |
| D. | Osmotic shock |
| Answer» D. Osmotic shock | |
| 2. |
__________ is a compound which is frequently used for DNA removal. |
| A. | 1.4% buffered sucrose |
| B. | Protamine sulphate |
| C. | Sodium hydroxide |
| D. | Lysozyme |
| Answer» C. Sodium hydroxide | |
| 3. |
Which of the following chemical is not used to isolate enzymes from cell free extract? |
| A. | Polyethyleneimine |
| B. | Polyvinylpyrrolidone |
| C. | EDTA |
| D. | Magnesium chloride |
| Answer» D. Magnesium chloride | |
| 4. |
Cetyl trimethyl ammonium bromide is a cationic detergent. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
The increase in pH affects the inter H2 bonds and intramolecular hydrophobic bonds are majorly affected leading to denaturation of DNA. |
| A. | True |
| B. | False |
| Answer» C. | |
| 6. |
Increase or decrease in _________ leads to protonation or deprotonation of bases which leads to melting of DNA in turn isolating the enzyme after centrifugation. |
| A. | temperature |
| B. | ionic strength |
| C. | pH |
| D. | detergent use |
| Answer» D. detergent use | |
| 7. |
___________ is responsible for maintaining biologically native structure of an enzyme and even nucleic acid. |
| A. | pH |
| B. | Proton |
| C. | ATP |
| D. | Electrons |
| Answer» C. ATP | |
| 8. |
Which one if the chemicals is not used to isolate enzymes from cell free extract? |
| A. | 1.4% buffered sucrose |
| B. | Cetyl trimethyl ammonium bromide |
| C. | Nucleases |
| D. | Streptomycin sulphate |
| Answer» B. Cetyl trimethyl ammonium bromide | |
| 9. |
Which of the following methods cannot be used to isolate enzymes from cell free extract? |
| A. | Use of factors like pH and ionic strength |
| B. | Use of chemicals |
| C. | Homogenization |
| D. | Use of temperature factor |
| Answer» D. Use of temperature factor | |
| 10. |
Enzymes can be isolated from cell free extract by which one of the following methods? |
| A. | Osmotic shock |
| B. | Alkali treatment |
| C. | Factors like pH, temperature and low ionic strength |
| D. | Ultrasonication |
| Answer» D. Ultrasonication | |
| 11. |
_________________ is the main contaminant present in the resultant free extract from cells. |
| A. | Chemicals |
| B. | Simple sugars |
| C. | Lipids |
| D. | Nucleic acids |
| Answer» E. | |