 
			 
			MCQOPTIONS
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				This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | In trans-splicing the excised intron forms a lariat structure. | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 2. | The transesterification reaction is ATP independent. | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 3. | The second transesterification reaction occurs between __________ | 
| A. | The 3’ splicing site of intron and 5’ splice site of intron | 
| B. | The 3’ splicing site of intron and the branch point site | 
| C. | The 5’ splicing site of intron and 3’ end of exon | 
| D. | The 5’ end of exon and the 3’ splice site | 
| Answer» E. | |
| 4. | The first transesterification reaction between the branch point site and the 5’ splice site is a __________ reaction. | 
| A. | SN1 | 
| B. | SN2 | 
| C. | E1 | 
| D. | E2 | 
| Answer» C. E1 | |
| 5. | The 3’ end of an intron is marked by __________ | 
| A. | Donor site | 
| B. | Acceptor site | 
| C. | AT rich site | 
| D. | Branch point site | 
| Answer» C. AT rich site | |
| 6. | THE_TRANSESTERIFICATION_REACTION_IS_ATP_INDEPENDENT.?$ | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 7. | In_trans-splicing_the_excised_intron_forms_a_lariat_structure.$ | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 8. | The second transesterification reaction occurs between _________? | 
| A. | The 3’ splicing site of intron and 5’ splice site of intron | 
| B. | The 3’ splicing site of intron and the branch point site | 
| C. | The 5’ splicing site of intron and 3’ end of exon | 
| D. | The 5’ end of exon and the 3’ splice site | 
| Answer» E. | |
| 9. | The first transesterification reaction between the branch point site and the 5’ splice site is a __________ reaction.$ | 
| A. | SN1 | 
| B. | SN2 | 
| C. | E1 | 
| D. | E2 | 
| Answer» C. E1 | |
| 10. | To achieve splicing of an intron how many phosphodiester bonds are formed __________ | 
| A. | 1 | 
| B. | 2 | 
| C. | 3 | 
| D. | 0 | 
| Answer» C. 3 | |
| 11. | How many consensus sequences for splicing are found in an exon? | 
| A. | 1 | 
| B. | 2 | 
| C. | 3 | 
| D. | 0 | 
| Answer» E. | |
| 12. | The highly conserved base at the branch point site is __________ | 
| A. | A | 
| B. | U | 
| C. | G | 
| D. | C | 
| Answer» B. U | |
| 13. | The 3’ end of an intron is marked by __________$ | 
| A. | Donor site | 
| B. | Acceptor site | 
| C. | AT rich site | 
| D. | Branch point site | 
| Answer» C. AT rich site | |
| 14. | During splicing which of the following sites is not recognized by the splicosome? | 
| A. | Donor | 
| B. | Branch point | 
| C. | Acceptor | 
| D. | G-rich site | 
| Answer» E. | |
| 15. | The splice site is found in __________ | 
| A. | 3’ end of exon | 
| B. | 5’ end of intron | 
| C. | Within the exon | 
| D. | Within the intron | 
| Answer» C. Within the exon | |