 
			 
			MCQOPTIONS
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				This section includes 195 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | The medium required for glutamic acid mainly consist of | 
| A. | glucose | 
| B. | molasses | 
| C. | sucrose | 
| D. | lactose | 
| Answer» B. molasses | |
| 2. | Which of the following species is used for vitamin B12 production by fermentation process? | 
| A. | Streptomyces griseus | 
| B. | Streptomyces olivaceus | 
| C. | Bacillus megaterium | 
| D. | All of these | 
| Answer» E. | |
| 3. | How much time is required to inject a copy of the whole Hfr E.coli genome? | 
| A. | 24 hrs | 
| B. | 30 mins | 
| C. | 100 mins | 
| D. | 48 hrs | 
| Answer» D. 48 hrs | |
| 4. | The viral DNA may be incorporated into the phage head during phage assembly. | 
| A. | True | 
| B. | False | 
| C. | May be | 
| D. | Can't say | 
| Answer» C. May be | |
| 5. | "Vinegar mother" in Orleans process is | 
| A. | inoculum of acetobacter bacteria used | 
| B. | considerable slime and film growth of Acetobacter bacteria on the surface of the alcoholic broth | 
| C. | cellulosic strands produced by Acetobacter bacteria to hold cells together | 
| D. | none of the above | 
| Answer» C. cellulosic strands produced by Acetobacter bacteria to hold cells together | |
| 6. | The submerged fermentation of vinegar utilizes special fermentor designs known as | 
| A. | acetator | 
| B. | cavitator | 
| C. | packed vinegar generator | 
| D. | both (a) and (b) | 
| Answer» E. | |
| 7. | Which of the following is not a principal media for industrial alcohol production by fermentation? | 
| A. | Black strap molasses | 
| B. | Sulphite waste liquor | 
| C. | Wood waste | 
| D. | Glucose | 
| Answer» E. | |
| 8. | Which of the following organism produces enzyme taka diastase? | 
| A. | A. oryzae | 
| B. | B. subtilis | 
| C. | A. niger | 
| D. | S. cerevisiae | 
| Answer» B. B. subtilis | |
| 9. | The organism B. brevis can be used commercially for the production of | 
| A. | gramicidin A | 
| B. | tyrothricin(bacitracin tyrocidin complex) | 
| C. | tyrocidin | 
| D. | all of the above | 
| Answer» E. | |
| 10. | The organisum used for production of streptomycin is | 
| A. | Streptomyces rimosus | 
| B. | Streptomyces griseus | 
| C. | Streptomyces aureofaciens | 
| D. | Streptomyces erythreus | 
| Answer» C. Streptomyces aureofaciens | |
| 11. | β-carotene is also known as | 
| A. | vitamin A | 
| B. | provitamin A | 
| C. | vitamin C | 
| D. | vitamin D | 
| Answer» C. vitamin C | |
| 12. | The penicillin F which was produced at first, required the mold | 
| A. | Penicillium chrysogenum | 
| B. | Pencillium notatum | 
| C. | Pencillium chrysogenum Q-176 | 
| D. | none of the above | 
| Answer» C. Pencillium chrysogenum Q-176 | |
| 13. | The 95% ethanol is equivalent to | 
| A. | 190 proof | 
| B. | 110 proof | 
| C. | 170 proof | 
| D. | 120 proof | 
| Answer» B. 110 proof | |
| 14. | The organism used to produce L-glutamic acid is | 
| A. | Micrococcus glutamicus | 
| B. | Brevibacterium aminogenes | 
| C. | Arthrobacter globiformis | 
| D. | all of these | 
| Answer» E. | |
| 15. | The DNA uptake process does not require any energy. | 
| A. | True | 
| B. | False | 
| C. | May be | 
| D. | Can't say | 
| Answer» C. May be | |
| 16. | The Hfr chromosome is transferred to the F— cell in a _____________ fashion. | 
| A. | circular | 
| B. | coiled | 
| C. | dimer | 
| D. | linear | 
| Answer» E. | |
| 17. | Malt used to ferment beer is prepared by | 
| A. | roasting barley | 
| B. | washing the barley with water and drying it in air | 
| C. | soaking barley in water followed by germination and drying | 
| D. | treating barley with bovine amylase followed by roasting | 
| Answer» D. treating barley with bovine amylase followed by roasting | |
| 18. | Bacteriophages inject their nucleic acid into the bacterium. | 
| A. | True | 
| B. | False | 
| C. | May be | 
| D. | Can't say | 
| Answer» B. False | |
| 19. | Which of the following micro organism utilizes lactose as a carbon source in production of lactic acid from whey media? | 
| A. | Lactobacillus bulgaricus | 
| B. | Lactobacillus delbrueckii | 
| C. | Lactobacillus pentosus | 
| D. | Leuconostoc mesenteroides | 
| Answer» B. Lactobacillus delbrueckii | |
| 20. | When viral genome can become integrated into the bacterial genome they are known as ____________ | 
| A. | temperate phage | 
| B. | prophage | 
| C. | bacteriophage | 
| D. | episome | 
| Answer» C. bacteriophage | |
| 21. | The early assay employed for measuring penicillin activity were based upon the quantity required to prevent growth of S. aureus over | 
| A. | 20 mm diameter zone using the cylinder plate method of assay | 
| B. | 24 mm diameter zone using the cylinder plate method of assay | 
| C. | 200 mm diameter zone using the cylinder plate method of assay | 
| D. | None of the above | 
| Answer» C. 200 mm diameter zone using the cylinder plate method of assay | |
| 22. | The transfer of naked DNA from one cell to another is referred to as _______________ | 
| A. | Transduction | 
| B. | Lysogeny | 
| C. | Transformation | 
| D. | Conjugation | 
| Answer» D. Conjugation | |
| 23. | The Candida species require fermentation equipment lined with plastic because they are extremely sensitive to | 
| A. | traces of cobalt | 
| B. | traces of nickel | 
| C. | traces of iron | 
| D. | none of these | 
| Answer» D. none of these | |
| 24. | Cephalosporin an antibiotic closely related to penicillin in structure and antibiotic activity are produced by species of | 
| A. | Cephalosporium | 
| B. | Cephalospodium | 
| C. | Pseudomonas | 
| D. | Bacillus | 
| Answer» C. Pseudomonas | |
| 25. | Who was the first scientist to observe the antibiotic and therapeutic properties of penicillin? | 
| A. | Louis Pasteur | 
| B. | Alexander Fleming | 
| C. | S. C. Prescott | 
| D. | E. P. Abraham | 
| Answer» C. S. C. Prescott | |
| 26. | In Corynebacterium glutamicum, tryptophan biosynthesis is regulated by the activity of | 
| A. | DAHP synthetase | 
| B. | Anthranilate synthase | 
| C. | Both (a) and (b) | 
| D. | Phosphoenol pyruvate | 
| Answer» D. Phosphoenol pyruvate | |
| 27. | The organism which utilizes the pentose of sulphite waste liquor for lactic acid production is | 
| A. | Streptococcus lactis | 
| B. | Lactobacillus bulgaricus | 
| C. | Lactobacillus casei | 
| D. | Lactobacillus pentosus | 
| Answer» E. | |
| 28. | The sugar concentration of molasses used in fermentation should be | 
| A. | 10-18% | 
| B. | 20-30% | 
| C. | 4-5% | 
| D. | 30-38% | 
| Answer» B. 20-30% | |
| 29. | The aerated submerged bacterial fermentation to produce vitamin B12 employs strains of | 
| A. | Propionibacterium | 
| B. | Pseudomonas | 
| C. | Acetobacter | 
| D. | both (a) and (b) | 
| Answer» E. | |
| 30. | Rickes et al. recovered active crystalline vitamin B12 from the culture of | 
| A. | Streptomyces griseus | 
| B. | Bacillus megaterium | 
| C. | Streptomyces olivaceus | 
| D. | Streptomyces lactis | 
| Answer» B. Bacillus megaterium | |
| 31. | Which of the following cephalosporin is more active than penicillin against various species of Salmonella? | 
| A. | Cephalosporin N | 
| B. | Cephalosporin C | 
| C. | Synnematin | 
| D. | Both (a) and (c) | 
| Answer» E. | |
| 32. | The enzyme which converts optically active isomers of lactic acid to the optically inactive racemic mixture is | 
| A. | isomerase | 
| B. | racemase | 
| C. | dehydrogenase | 
| D. | invertase | 
| Answer» C. dehydrogenase | |
| 33. | Which of the following things was identified as the transforming principle? | 
| A. | DNA | 
| B. | RNA | 
| C. | Proteins | 
| D. | Carbohydrates | 
| Answer» B. RNA | |
| 34. | In Griffith’s experiment which of the following strains of pneumococci was isolated from dead mice? | 
| A. | live rough cells | 
| B. | dead rough cells | 
| C. | live smooth cells | 
| D. | dead smooth cells | 
| Answer» D. dead smooth cells | |
| 35. | The major organism used in the microbial production of citric acid is | 
| A. | Penicillin notatum | 
| B. | Rhizopus nigrificins | 
| C. | Aspergillus niger | 
| D. | Lactobacillis delbrueckii | 
| Answer» D. Lactobacillis delbrueckii | |
| 36. | A major organism used in commercial bioleaching for copper recovery is | 
| A. | Desulfovibrio desulfuricans | 
| B. | Pseudomonas aeruginosa | 
| C. | Aspergillus niger | 
| D. | Thiobacillus ferrooxidans | 
| Answer» E. | |
| 37. | Which of the following are commercially used for bacterial amylases production? | 
| A. | Bacillus subtilis | 
| B. | Bacillus diastaticus | 
| C. | Clostridium acetobutylicum | 
| D. | Both (a) and (b) | 
| Answer» E. | |
| 38. | Penicillin was first produced commercially using | 
| A. | stationary mat culture | 
| B. | deep tank aerated fermentations | 
| C. | continuous fermentation | 
| D. | none of the above | 
| Answer» B. deep tank aerated fermentations | |
| 39. | The first commercial fermention carried out to obtain L-lysine is known as | 
| A. | dual fermentation | 
| B. | indirect fermentation | 
| C. | direct fermentation | 
| D. | both (a) and (b) | 
| Answer» E. | |
| 40. | The medium utilized for fungal citric acid production should be deficient in | 
| A. | phosphate | 
| B. | manganese | 
| C. | iron | 
| D. | all of these | 
| Answer» E. | |
| 41. | Bacillus thuringiensis is useful as a bacterial | 
| A. | source of antibiotics | 
| B. | producer of vitamins | 
| C. | herbicide | 
| D. | pesticide | 
| Answer» E. | |
| 42. | Which of the following can be used as a measure to construct a linkage map of the Hfr chromosome? | 
| A. | frequency of recombination | 
| B. | time of entry | 
| C. | locus of mutation | 
| D. | transfer of F factor | 
| Answer» C. locus of mutation | |
| 43. | The present day high yielding penicillin are obtained from | 
| A. | Penicillium chrysogenum (NRRL, 1951) | 
| B. | Penicillium chrysogenum (Q-176) | 
| C. | Penicillium chrysogenum (Q-179) | 
| D. | none of the above | 
| Answer» C. Penicillium chrysogenum (Q-179) | |
| 44. | Which of the following is known for greater stability of the riboflavin producing capacity? | 
| A. | Eremothecium ashbyii | 
| B. | Ashbya gossypii | 
| C. | Candida flareri | 
| D. | None of these | 
| Answer» C. Candida flareri | |
| 45. | The beet molasses is pretreated with __________ to reduce great quantity of trace metals. | 
| A. | ferrocyanide or ferricyanide | 
| B. | ethylenediamine tetrachloro acetic acid | 
| C. | hydrochloric acid | 
| D. | sulphuric acid | 
| Answer» B. ethylenediamine tetrachloro acetic acid | |
| 46. | The cell in which the F factor carries along with it some chromosomal genes are known as ____________ | 
| A. | F+ cell | 
| B. | F— cell | 
| C. | F’ cell | 
| D. | F’’’ cell | 
| Answer» D. F’’’ cell | |
| 47. | How many percent of thr+ transducing phages also carry leu+? | 
| A. | 10% | 
| B. | 5% | 
| C. | 3% | 
| D. | 15% | 
| Answer» D. 15% | |
| 48. | Which of the following is true for an Hfr X F— cross? | 
| A. | frequency of recombination high,transfer of F factor low | 
| B. | frequency of recombination high,transfer of F factor high | 
| C. | frequency of recombination low,transfer of F factor high | 
| D. | frequency of recombination low,transfer of F factor low | 
| Answer» B. frequency of recombination high,transfer of F factor high | |
| 49. | The recovery of vitamin from fermentation broth is carried out prior to | 
| A. | acidification | 
| B. | alcohol treatment | 
| C. | autolysis | 
| D. | none of these | 
| Answer» D. none of these | |
| 50. | Genetic recombination occurs between homologous chromosomes. | 
| A. | True | 
| B. | False | 
| C. | May be | 
| D. | Can't say | 
| Answer» B. False | |