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This section includes 195 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The medium required for glutamic acid mainly consist of |
| A. | glucose |
| B. | molasses |
| C. | sucrose |
| D. | lactose |
| Answer» B. molasses | |
| 2. |
Which of the following species is used for vitamin B12 production by fermentation process? |
| A. | Streptomyces griseus |
| B. | Streptomyces olivaceus |
| C. | Bacillus megaterium |
| D. | All of these |
| Answer» E. | |
| 3. |
How much time is required to inject a copy of the whole Hfr E.coli genome? |
| A. | 24 hrs |
| B. | 30 mins |
| C. | 100 mins |
| D. | 48 hrs |
| Answer» D. 48 hrs | |
| 4. |
The viral DNA may be incorporated into the phage head during phage assembly. |
| A. | True |
| B. | False |
| C. | May be |
| D. | Can't say |
| Answer» C. May be | |
| 5. |
"Vinegar mother" in Orleans process is |
| A. | inoculum of acetobacter bacteria used |
| B. | considerable slime and film growth of Acetobacter bacteria on the surface of the alcoholic broth |
| C. | cellulosic strands produced by Acetobacter bacteria to hold cells together |
| D. | none of the above |
| Answer» C. cellulosic strands produced by Acetobacter bacteria to hold cells together | |
| 6. |
The submerged fermentation of vinegar utilizes special fermentor designs known as |
| A. | acetator |
| B. | cavitator |
| C. | packed vinegar generator |
| D. | both (a) and (b) |
| Answer» E. | |
| 7. |
Which of the following is not a principal media for industrial alcohol production by fermentation? |
| A. | Black strap molasses |
| B. | Sulphite waste liquor |
| C. | Wood waste |
| D. | Glucose |
| Answer» E. | |
| 8. |
Which of the following organism produces enzyme taka diastase? |
| A. | A. oryzae |
| B. | B. subtilis |
| C. | A. niger |
| D. | S. cerevisiae |
| Answer» B. B. subtilis | |
| 9. |
The organism B. brevis can be used commercially for the production of |
| A. | gramicidin A |
| B. | tyrothricin(bacitracin tyrocidin complex) |
| C. | tyrocidin |
| D. | all of the above |
| Answer» E. | |
| 10. |
The organisum used for production of streptomycin is |
| A. | Streptomyces rimosus |
| B. | Streptomyces griseus |
| C. | Streptomyces aureofaciens |
| D. | Streptomyces erythreus |
| Answer» C. Streptomyces aureofaciens | |
| 11. |
β-carotene is also known as |
| A. | vitamin A |
| B. | provitamin A |
| C. | vitamin C |
| D. | vitamin D |
| Answer» C. vitamin C | |
| 12. |
The penicillin F which was produced at first, required the mold |
| A. | Penicillium chrysogenum |
| B. | Pencillium notatum |
| C. | Pencillium chrysogenum Q-176 |
| D. | none of the above |
| Answer» C. Pencillium chrysogenum Q-176 | |
| 13. |
The 95% ethanol is equivalent to |
| A. | 190 proof |
| B. | 110 proof |
| C. | 170 proof |
| D. | 120 proof |
| Answer» B. 110 proof | |
| 14. |
The organism used to produce L-glutamic acid is |
| A. | Micrococcus glutamicus |
| B. | Brevibacterium aminogenes |
| C. | Arthrobacter globiformis |
| D. | all of these |
| Answer» E. | |
| 15. |
The DNA uptake process does not require any energy. |
| A. | True |
| B. | False |
| C. | May be |
| D. | Can't say |
| Answer» C. May be | |
| 16. |
The Hfr chromosome is transferred to the F— cell in a _____________ fashion. |
| A. | circular |
| B. | coiled |
| C. | dimer |
| D. | linear |
| Answer» E. | |
| 17. |
Malt used to ferment beer is prepared by |
| A. | roasting barley |
| B. | washing the barley with water and drying it in air |
| C. | soaking barley in water followed by germination and drying |
| D. | treating barley with bovine amylase followed by roasting |
| Answer» D. treating barley with bovine amylase followed by roasting | |
| 18. |
Bacteriophages inject their nucleic acid into the bacterium. |
| A. | True |
| B. | False |
| C. | May be |
| D. | Can't say |
| Answer» B. False | |
| 19. |
Which of the following micro organism utilizes lactose as a carbon source in production of lactic acid from whey media? |
| A. | Lactobacillus bulgaricus |
| B. | Lactobacillus delbrueckii |
| C. | Lactobacillus pentosus |
| D. | Leuconostoc mesenteroides |
| Answer» B. Lactobacillus delbrueckii | |
| 20. |
When viral genome can become integrated into the bacterial genome they are known as ____________ |
| A. | temperate phage |
| B. | prophage |
| C. | bacteriophage |
| D. | episome |
| Answer» C. bacteriophage | |
| 21. |
The early assay employed for measuring penicillin activity were based upon the quantity required to prevent growth of S. aureus over |
| A. | 20 mm diameter zone using the cylinder plate method of assay |
| B. | 24 mm diameter zone using the cylinder plate method of assay |
| C. | 200 mm diameter zone using the cylinder plate method of assay |
| D. | None of the above |
| Answer» C. 200 mm diameter zone using the cylinder plate method of assay | |
| 22. |
The transfer of naked DNA from one cell to another is referred to as _______________ |
| A. | Transduction |
| B. | Lysogeny |
| C. | Transformation |
| D. | Conjugation |
| Answer» D. Conjugation | |
| 23. |
The Candida species require fermentation equipment lined with plastic because they are extremely sensitive to |
| A. | traces of cobalt |
| B. | traces of nickel |
| C. | traces of iron |
| D. | none of these |
| Answer» D. none of these | |
| 24. |
Cephalosporin an antibiotic closely related to penicillin in structure and antibiotic activity are produced by species of |
| A. | Cephalosporium |
| B. | Cephalospodium |
| C. | Pseudomonas |
| D. | Bacillus |
| Answer» C. Pseudomonas | |
| 25. |
Who was the first scientist to observe the antibiotic and therapeutic properties of penicillin? |
| A. | Louis Pasteur |
| B. | Alexander Fleming |
| C. | S. C. Prescott |
| D. | E. P. Abraham |
| Answer» C. S. C. Prescott | |
| 26. |
In Corynebacterium glutamicum, tryptophan biosynthesis is regulated by the activity of |
| A. | DAHP synthetase |
| B. | Anthranilate synthase |
| C. | Both (a) and (b) |
| D. | Phosphoenol pyruvate |
| Answer» D. Phosphoenol pyruvate | |
| 27. |
The organism which utilizes the pentose of sulphite waste liquor for lactic acid production is |
| A. | Streptococcus lactis |
| B. | Lactobacillus bulgaricus |
| C. | Lactobacillus casei |
| D. | Lactobacillus pentosus |
| Answer» E. | |
| 28. |
The sugar concentration of molasses used in fermentation should be |
| A. | 10-18% |
| B. | 20-30% |
| C. | 4-5% |
| D. | 30-38% |
| Answer» B. 20-30% | |
| 29. |
The aerated submerged bacterial fermentation to produce vitamin B12 employs strains of |
| A. | Propionibacterium |
| B. | Pseudomonas |
| C. | Acetobacter |
| D. | both (a) and (b) |
| Answer» E. | |
| 30. |
Rickes et al. recovered active crystalline vitamin B12 from the culture of |
| A. | Streptomyces griseus |
| B. | Bacillus megaterium |
| C. | Streptomyces olivaceus |
| D. | Streptomyces lactis |
| Answer» B. Bacillus megaterium | |
| 31. |
Which of the following cephalosporin is more active than penicillin against various species of Salmonella? |
| A. | Cephalosporin N |
| B. | Cephalosporin C |
| C. | Synnematin |
| D. | Both (a) and (c) |
| Answer» E. | |
| 32. |
The enzyme which converts optically active isomers of lactic acid to the optically inactive racemic mixture is |
| A. | isomerase |
| B. | racemase |
| C. | dehydrogenase |
| D. | invertase |
| Answer» C. dehydrogenase | |
| 33. |
Which of the following things was identified as the transforming principle? |
| A. | DNA |
| B. | RNA |
| C. | Proteins |
| D. | Carbohydrates |
| Answer» B. RNA | |
| 34. |
In Griffith’s experiment which of the following strains of pneumococci was isolated from dead mice? |
| A. | live rough cells |
| B. | dead rough cells |
| C. | live smooth cells |
| D. | dead smooth cells |
| Answer» D. dead smooth cells | |
| 35. |
The major organism used in the microbial production of citric acid is |
| A. | Penicillin notatum |
| B. | Rhizopus nigrificins |
| C. | Aspergillus niger |
| D. | Lactobacillis delbrueckii |
| Answer» D. Lactobacillis delbrueckii | |
| 36. |
A major organism used in commercial bioleaching for copper recovery is |
| A. | Desulfovibrio desulfuricans |
| B. | Pseudomonas aeruginosa |
| C. | Aspergillus niger |
| D. | Thiobacillus ferrooxidans |
| Answer» E. | |
| 37. |
Which of the following are commercially used for bacterial amylases production? |
| A. | Bacillus subtilis |
| B. | Bacillus diastaticus |
| C. | Clostridium acetobutylicum |
| D. | Both (a) and (b) |
| Answer» E. | |
| 38. |
Penicillin was first produced commercially using |
| A. | stationary mat culture |
| B. | deep tank aerated fermentations |
| C. | continuous fermentation |
| D. | none of the above |
| Answer» B. deep tank aerated fermentations | |
| 39. |
The first commercial fermention carried out to obtain L-lysine is known as |
| A. | dual fermentation |
| B. | indirect fermentation |
| C. | direct fermentation |
| D. | both (a) and (b) |
| Answer» E. | |
| 40. |
The medium utilized for fungal citric acid production should be deficient in |
| A. | phosphate |
| B. | manganese |
| C. | iron |
| D. | all of these |
| Answer» E. | |
| 41. |
Bacillus thuringiensis is useful as a bacterial |
| A. | source of antibiotics |
| B. | producer of vitamins |
| C. | herbicide |
| D. | pesticide |
| Answer» E. | |
| 42. |
Which of the following can be used as a measure to construct a linkage map of the Hfr chromosome? |
| A. | frequency of recombination |
| B. | time of entry |
| C. | locus of mutation |
| D. | transfer of F factor |
| Answer» C. locus of mutation | |
| 43. |
The present day high yielding penicillin are obtained from |
| A. | Penicillium chrysogenum (NRRL, 1951) |
| B. | Penicillium chrysogenum (Q-176) |
| C. | Penicillium chrysogenum (Q-179) |
| D. | none of the above |
| Answer» C. Penicillium chrysogenum (Q-179) | |
| 44. |
Which of the following is known for greater stability of the riboflavin producing capacity? |
| A. | Eremothecium ashbyii |
| B. | Ashbya gossypii |
| C. | Candida flareri |
| D. | None of these |
| Answer» C. Candida flareri | |
| 45. |
The beet molasses is pretreated with __________ to reduce great quantity of trace metals. |
| A. | ferrocyanide or ferricyanide |
| B. | ethylenediamine tetrachloro acetic acid |
| C. | hydrochloric acid |
| D. | sulphuric acid |
| Answer» B. ethylenediamine tetrachloro acetic acid | |
| 46. |
The cell in which the F factor carries along with it some chromosomal genes are known as ____________ |
| A. | F+ cell |
| B. | F— cell |
| C. | F’ cell |
| D. | F’’’ cell |
| Answer» D. F’’’ cell | |
| 47. |
How many percent of thr+ transducing phages also carry leu+? |
| A. | 10% |
| B. | 5% |
| C. | 3% |
| D. | 15% |
| Answer» D. 15% | |
| 48. |
Which of the following is true for an Hfr X F— cross? |
| A. | frequency of recombination high,transfer of F factor low |
| B. | frequency of recombination high,transfer of F factor high |
| C. | frequency of recombination low,transfer of F factor high |
| D. | frequency of recombination low,transfer of F factor low |
| Answer» B. frequency of recombination high,transfer of F factor high | |
| 49. |
The recovery of vitamin from fermentation broth is carried out prior to |
| A. | acidification |
| B. | alcohol treatment |
| C. | autolysis |
| D. | none of these |
| Answer» D. none of these | |
| 50. |
Genetic recombination occurs between homologous chromosomes. |
| A. | True |
| B. | False |
| C. | May be |
| D. | Can't say |
| Answer» B. False | |