Explore topic-wise MCQs in Bioprocess Engineering.

This section includes 9 Mcqs, each offering curated multiple-choice questions to sharpen your Bioprocess Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

Immobilised lactase is used to hydrolyse lactose in dairy waste to glucose and galactose. Enzyme is immobilised in resin particles and packed into a 0.5 m3 column. The total effectiveness factor for the system is close to unity; Km for the immobilised enzyme is 1.32 kg m-3; Vmax is 45 kg m-3 h-1. The lactose concentration in the feed stream is 9.5 kg m-3; a substrate conversion of 98% is required. The column is operated with plug flow for a total of 310 d per year. At what flow rate should the reactor be operated?

A. 1.56 m<sup>3</sup> h<sup>-1</sup>
B. 1.58 m<sup>3</sup> h<sup>-1</sup>
C. 1.50 m<sup>3</sup> h<sup>-1</sup>
D. 1.54 m<sup>3</sup> h<sup>-1</sup>
Answer» B. 1.58 m<sup>3</sup> h<sup>-1</sup>
Discussion
2.

By taking Q10 and at the flow rate of Q10, what is the cell density?

A. 0.42 g l<sup>-1</sup>
B. 0.44 g l<sup>-1</sup>
C. 0.46 g l<sup>-1</sup>
D. 0.48 g l<sup>-1</sup>
Answer» B. 0.44 g l<sup>-1</sup>
Discussion
3.

The Zymomonas mobilis cells are used for chemostat culture in a 60 m3 fermenter. The feed contains 12 g l-1 glucose; Ks for the organism is 0.2 g l-1. What flow rate is required for a steady-state substrate concentration of 1.5 g l-1?

A. 15.6 m<sup>3</sup> h<sup>-1</sup>
B. 15.8 m<sup>3</sup> h<sup>-1</sup>
C. 15.4 m<sup>3</sup> h<sup>-1</sup>
D. 15.2 m<sup>3</sup> h<sup>-1</sup>
Answer» B. 15.8 m<sup>3</sup> h<sup>-1</sup>
Discussion
4.

By taking the parameters question 8 and question 7, determine batch culture times required to produce 100 g ethanol.

A. 3.2 h
B. 3.4 h
C. 3.6 h
D. 3.8 h
Answer» C. 3.6 h
Discussion
5.

By taking the pararmeters of Q7 and Determine batch culture times required to achieve 90% substrate conversion.

A. 5.1 h
B. 5.3 h
C. 5.5 h
D. 5.7 h
Answer» E.
Discussion
6.

Zymomonas mobilis is used to convert glucose to ethanol in a batch fermenter under anaerobic conditions. The yield of biomass from substrate is 0.06 g g-1; YPX is 7.7 g g-1. The maintenance coefficient is 2.2 g g-1 h-1; the specific rate of product formation due to maintenance is 1.1 h-1. The maximum specific growth rate of Z. mobilis is approximately 0.3 h-1.5 g bacteria are inoculated into 50 litres of medium containing 12 g l-1 glucose. Determine batch culture times required to produce 10 g biomass.

A. 3.1 h
B. 3.3 h
C. 3.5 h
D. 3.7 h
Answer» E.
Discussion
7.

By taking the parameters of Q5, and the batch reaction time tb.

A. 5.0 h
B. 10.0 h
C. 15.0 h
D. 20.0 h
Answer» B. 10.0 h
Discussion
8.

In batch reaction time with enzyme deactivation, calculate the first-order deactivation rate constant.
(Given so = 12 mM; vmax = 9 mM h-1; Km = 8.9 mM; sf = 1.2 mM; th = 4.4 h).

A. 0.150 h<sup>-1</sup>
B. 0.158 h<sup>-1</sup>
C. 0.155 h<sup>-1</sup>
D. 0.154 h<sup>-1</sup>
Answer» C. 0.155 h<sup>-1</sup>
Discussion
9.

Convert vmax = 7 mmol m-3 s-1 into mM h-1.

A. 25
B. 20
C. 25.20
D. 20.25
Answer» D. 20.25
Discussion