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This section includes 23 Mcqs, each offering curated multiple-choice questions to sharpen your Bioprocess Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Immobilised lactase is used to hydrolyse lactose in dairy waste to glucose and galactose. Enzyme is immobilised in resin particles and packed into a 0.5 m3 column. The total effectiveness factor for the system is close to unity; Km for the immobilised enzyme is 1.32 kg m-3; Vmax is 45 kg m-3 h-1. The lactose concentration in the feed stream is 9.5 kg m-3; a substrate conversion of 98% is required. The column is operated with plug flow for a total of 310 d per year. At what flow rate should the reactor be operated? |
| A. | 1.56 m3 h-1 |
| B. | 1.58 m3 h-1 |
| C. | 1.50 m3 h-1 |
| D. | 1.54 m3 h-1 |
| Answer» B. 1.58 m3 h-1 | |
| 2. |
By taking Q10 and at the flow rate of Q10, what is the cell density? |
| A. | 0.42 g l-1 |
| B. | 0.44 g l-1 |
| C. | 0.46 g l-1 |
| D. | 0.48 g l-1 |
| Answer» B. 0.44 g l-1 | |
| 3. |
The Zymomonas mobilis cells are used for chemostat culture in a 60 m3 fermenter. The feed contains 12 g l-1 glucose; Ks for the organism is 0.2 g l-1. What flow rate is required for a steady-state substrate concentration of 1.5 g l-1? |
| A. | 15.6 m3 h-1 |
| B. | 15.8 m3 h-1 |
| C. | 15.4 m3 h-1 |
| D. | 15.2 m3 h-1 |
| Answer» B. 15.8 m3 h-1 | |
| 4. |
By taking the parameters question 8 and question 7, determine batch culture times required to produce 100 g ethanol. |
| A. | 3.2 h |
| B. | 3.4 h |
| C. | 3.6 h |
| D. | 3.8 h |
| Answer» C. 3.6 h | |
| 5. |
By taking the pararmeters of Q7 and Determine batch culture times required to achieve 90% substrate conversion. |
| A. | 5.1 h |
| B. | 5.3 h |
| C. | 5.5 h |
| D. | 5.7 h |
| Answer» E. | |
| 6. |
Zymomonas mobilis is used to convert glucose to ethanol in a batch fermenter under anaerobic conditions. The yield of biomass from substrate is 0.06 g g-1; YPX is 7.7 g g-1. The maintenance coefficient is 2.2 g g-1 h-1; the specific rate of product formation due to maintenance is 1.1 h-1. The maximum specific growth rate of Z. mobilis is approximately 0.3 h-1.5 g bacteria are inoculated into 50 litres of medium containing 12 g l-1 glucose. Determine batch culture times required to produce 10 g biomass. |
| A. | 3.1 h |
| B. | 3.3 h |
| C. | 3.5 h |
| D. | 3.7 h |
| Answer» E. | |
| 7. |
In batch reaction time with enzyme deactivation, calculate the first-order deactivation rate constant.(Given – so = 12 mM; vmax = 9 mM h-1; Km = 8.9 mM; sf = 1.2 mM; th = 4.4 h). |
| A. | 0.150 h-1 |
| B. | 0.158 h-1 |
| C. | 0.155 h-1 |
| D. | 0.154 h-1 |
| Answer» C. 0.155 h-1 | |
| 8. |
Convert vmax = 7 mmol m-3 s-1 into mM h-1. |
| A. | 25 |
| B. | 20 |
| C. | 25.20 |
| D. | 20.25 |
| Answer» D. 20.25 | |
| 9. |
Convert vmax = 2.5 mmol m-3 s-1 into mM h-1. |
| A. | 2500 |
| B. | 900 |
| C. | 25 |
| D. | 9 |
| Answer» E. | |
| 10. |
BY_TAKING_THE_PARAMETERS_QUESTION_8_AND_QUESTION_7,_DETERMINE_BATCH_CULTURE_TIMES_REQUIRED_TO_PRODUCE_100_G_ETHANOL.?$ |
| A. | 3.2 h |
| B. | 3.4 h |
| C. | 3.6 h |
| D. | 3.8 h |
| Answer» C. 3.6 h | |
| 11. |
By taking Q10 and at the flow rate of Q10, what is the cell density?$ |
| A. | 0.42 g l<sup>-1</sup> |
| B. | 0.44 g l<sup>-1</sup> |
| C. | 0.46 g l<sup>-1</sup> |
| D. | 0.48 g l<sup>-1</sup> |
| Answer» B. 0.44 g l<sup>-1</sup> | |
| 12. |
The_Zymomonas_mobilis_cells_are_used_for_chemostat_culture_in_a_60_m3_fermenter._The_feed_contains_12_g_l-1_glucose;_Ks_for_the_organism_is_0.2_g_l-1._What_flow_rate_is_required_for_a_steady-state_substrate_concentration_of_1.5_g_l-1?$ |
| A. | 15.6 m<sup>3</sup> h<sup>-1</sup> |
| B. | 15.8 m<sup>3</sup> h<sup>-1</sup> |
| C. | 15.4 m<sup>3</sup> h<sup>-1</sup> |
| D. | 15.2 m<sup>3</sup> h<sup>-1</sup> |
| Answer» B. 15.8 m<sup>3</sup> h<sup>-1</sup> | |
| 13. |
By taking the parameters of Q14 into consideration, estimate how many tonnes of glucose are produced per year? |
| A. | 56.3 tonnes yr<sup>-1</sup> |
| B. | 56.6 tonnes yr<sup>-1</sup> |
| C. | 56.7 tonnes yr<sup>-1</sup> |
| D. | 56.5 tonnes yr<sup>-1</sup> |
| Answer» D. 56.5 tonnes yr<sup>-1</sup> | |
| 14. |
Immobilised lactase is used to hydrolyse lactose in dairy waste to glucose and galactose. Enzyme is immobilised in resin particles and packed into a 0.5 m3 column. The total effectiveness factor for the system is close to unity; Km for the immobilised enzyme is 1.32 kg m-3; Vmax is 45 kg m-3 h-1. The lactose concentration in the feed stream is 9.5 kg m-3; a substrate conversion of 98% is required. The column is operated with plug flow for a total of 310 d per year. At what flow rate should the reactor be operated? |
| A. | 1.56 m<sup>3</sup> hh<sup>-1</sup> |
| B. | 1.58 m<sup>3</sup> hh<sup>-1</sup> |
| C. | 1.50 m<sup>3</sup> h-1 |
| D. | 1.54 m<sup>3</sup> h-1 |
| Answer» B. 1.58 m<sup>3</sup> hh<sup>-1</sup> | |
| 15. |
Which of the following type is of the perfusion culture? |
| A. | Batch |
| B. | Conc. Batch |
| C. | Continuous |
| D. | Conc. Fed-Batch |
| Answer» E. | |
| 16. |
By taking Q10 and Q11 into account and at the flow rate of Q10, what concentration of ethanol is produced? |
| A. | 5.1 g l<sup>-1</sup> |
| B. | 5.3 g l<sup>-1</sup> |
| C. | 5.5 g l<sup>-1</sup> |
| D. | 5.7 g l<sup>-1</sup> |
| Answer» D. 5.7 g l<sup>-1</sup> | |
| 17. |
By taking the pararmeters of Q7 and Determine batch culture times required to achieve 90% substrate conversion? |
| A. | 5.1 h |
| B. | 5.3 h |
| C. | 5.5 h |
| D. | 5.7 h |
| Answer» E. | |
| 18. |
Zymomonas mobilis is used to convert glucose to ethanol in a batch fermenter under anaerobic conditions. The yield of biomass from substrate is 0.06 g g- 1; YPX is 7.7 g g- 1. The maintenance coefficient is 2.2 g g-1 h-1; the specific rate of product formation due to maintenance is 1.1 h-1. The maximum specific growth rate of Z. mobilis is approximately 0.3 h-1.5 g bacteria are inoculated into 50 litres of medium containing 12 g l-1 glucose. Determine batch culture times required to produce 10 g biomass. |
| A. | 3.1 h |
| B. | 3.3 h |
| C. | 3.5 h |
| D. | 3.7 h |
| Answer» E. | |
| 19. |
By taking the parameters of Q5, and the batch reaction time tb. |
| A. | 5.0 h |
| B. | 10.0 h |
| C. | 15.0 h |
| D. | 20.0 h |
| Answer» B. 10.0 h | |
| 20. |
In batch reaction time with enzyme deactivation, calculate the first-order deactivation rate constant. |
| A. | . |
| B. | 0.150 h<sup>-1</sup> |
| C. | 0.158 h<sup>-1</sup> |
| D. | 0.155 h<sup>-1</sup> |
| Answer» C. 0.158 h<sup>-1</sup> | |
| 21. |
Convert vmax = 2.5 mmol m-3 s-1 into mM h-1. |
| A. | 2500 |
| B. | 900 |
| C. | 25 |
| D. | 9 |
| Answer» E. | |
| 22. |
In a perfectly mixed reactor _________ |
| A. | The output composition is different from input composition |
| B. | The output composition is identical from input composition |
| C. | Both output and input composition are constant |
| D. | Both output and input composition are not constant |
| Answer» C. Both output and input composition are constant | |
| 23. |
Aerobic reactions are not batch operations. |
| A. | True |
| B. | False |
| Answer» B. False | |