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This section includes 60 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Generation time of is |
| A. | 20 minutes |
| B. | 20 hours |
| C. | 20 days |
| D. | 200 hours |
| Answer» B. 20 hours | |
| 2. |
The total biomass of an organism will be determined by the nutrient present in the lowest concentration relative to the organism's requirements is a statement of |
| A. | Liebig's law of the minimum |
| B. | Shelford's law of tolerance |
| C. | quorum sensing |
| D. | Heisenberg's principle of uncertainty |
| Answer» B. Shelford's law of tolerance | |
| 3. |
All organisms require at least small amounts of carbondioxide, However, some can use CO as their sole source of carbon. Such organisms are termed as |
| A. | autotrophs |
| B. | phototrophs |
| C. | chemotrophs |
| D. | photo-organotrophs |
| Answer» B. phototrophs | |
| 4. |
The ability of to produce bioluminescence chemicals only when a certain population density has been reached is an example of |
| A. | Liebig's law of the minimum |
| B. | Shelford's law of tolerance |
| C. | quorum sensing |
| D. | Heisenberg's principle of uncertainty |
| Answer» D. Heisenberg's principle of uncertainty | |
| 5. |
can grow as |
| A. | chemo-lithotrophs |
| B. | chemo-organotrophs |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» D. none of these | |
| 6. |
The combination of low levels of NaCl, NaNO (sodium nitrate), and slightly acid pH can prevent multiplication and toxin formation of |
| A. | all of these |
| Answer» E. | |
| 7. |
An organism is completely dependent on atmospheric O for growth. This organism is |
| A. | osmotolerant |
| B. | acidophile |
| C. | facultative anaerobe |
| D. | obligate aerobe |
| Answer» E. | |
| 8. |
Bacteria of genus use __________ as their electron source. |
| A. | ammonia |
| B. | H |
| C. | S |
| D. | succinate |
| E. | light |
| Answer» B. H | |
| 9. |
Which of the following is not the characteristic of a growth curve? |
| A. | Shows development of microbial population under relatively stable environmental conditions |
| B. | Plotted with logarithmic numbers |
| C. | Graphs numbers of microbes versus time |
| D. | Each growth curve consists of four distinct phases |
| Answer» E. | |
| 10. |
The term facultative anaerobe refers to an organism that |
| A. | doesn't use oxygen but tolerates it |
| B. | is killed by oxygen |
| C. | uses oxygen when present or grows without oxygen when absent |
| D. | requires less oxygen than is present in air |
| Answer» D. requires less oxygen than is present in air | |
| 11. |
Generation time of Escherichia coli is |
| A. | 20 minutes |
| B. | 20 hours |
| C. | 20 days |
| D. | 200 hours |
| Answer» B. 20 hours | |
| 12. |
Generation time is |
| A. | time required for the population to double |
| B. | time required for the initial adjustment |
| C. | obtained by expression t/n, where t = time interval, n = number of generation |
| D. | both (a) and (c) |
| Answer» E. | |
| 13. |
The microorganisms that grow best in a low-oxygen environment is called a (n) |
| A. | aerobe |
| B. | anaerobe |
| C. | facultative |
| D. | microaerophile |
| Answer» E. | |
| 14. |
Pseudomonas pseudoflava can grow as |
| A. | chemo-lithotrophs |
| B. | chemo-organotrophs |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» D. none of these | |
| 15. |
Which of the following has more tolerance for acidic pH (lower pH)? |
| A. | Yeast and moulds |
| B. | Bacteria |
| C. | <i>E. coli</i> |
| D. | None of these |
| Answer» B. Bacteria | |
| 16. |
Antimicrobial constituents for the microbial growth in foods are |
| A. | intrinsic factor |
| B. | extrinsic factor |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» B. extrinsic factor | |
| 17. |
The average size of cells during the exponential phase of growth is |
| A. | greater than the lag phase |
| B. | lesser than the lag phase |
| C. | equal to the lag phase |
| D. | none of these |
| Answer» C. equal to the lag phase | |
| 18. |
A culture broth tube was very turbid at the surface but clear throughout the rest of the tube indicating that the |
| A. | organism are aerobes |
| B. | organism should be grown in an anaerobic chamber |
| C. | organism cannot produce superoxide dismutase and/or catalase |
| D. | organism cannot tolerate oxygen |
| Answer» B. organism should be grown in an anaerobic chamber | |
| 19. |
The reproduction by budding occur in |
| A. | <i>Rhodopseudomonas acidophila</i> |
| B. | <i> Hyphomicrobium vulgare</i> |
| C. | <i>Bacillus subtilis</i> |
| D. | Both (a) and (b) |
| Answer» E. | |
| 20. |
A spore differs from an actively replicating bacterium in that the spore |
| A. | is produced during a process involving asymmetric division |
| B. | is able to withstand more extreme conditions than the replicating cell |
| C. | is metabolically inactive |
| D. | all of the above |
| Answer» E. | |
| 21. |
Bacteria and fungi multiply best |
| A. | below 16 C |
| B. | between 16-38 C |
| C. | above 38 C |
| D. | none of these |
| Answer» C. above 38 C | |
| 22. |
An organism has an optimal growth rate when the hydrogen ion concentration is very high. This organism is |
| A. | osmotolerant |
| B. | acidophile |
| C. | neutrophile |
| D. | aerotolerant anaerobe |
| Answer» C. neutrophile | |
| 23. |
Which of the following procedures uses a photocell to measure absorbance of a culture to regulate the flow of culture media? |
| A. | Coulter Counter |
| B. | Hemostat |
| C. | Petroff-Hausser chamber |
| D. | Trubidostat |
| Answer» E. | |
| 24. |
Organisms, using organic compounds as electron donors are called |
| A. | lithotrophs |
| B. | phototrophs |
| C. | chemotrophs |
| D. | organotrophs |
| Answer» E. | |
| 25. |
The total biomass of an organism will be determined by the nutrient present in the lowest concentration relative to the organism's requirements is a statement of |
| A. | Liebig's law of the minimum |
| B. | Shelford's law of tolerance |
| C. | quorum sensing |
| D. | Heisenberg's principle of uncertainty |
| Answer» B. Shelford's law of tolerance | |
| 26. |
Which of the following organisms typically get their carbon for biosynthesis from carbon dioxide? |
| A. | Glucose-fermenting bacteria (fermentation) |
| B. | Anaerobic, glucose-respiring bacteria (anaerobic respiration) |
| C. | Aerobic, glucose-respiring bacteria (aerobic respiration) |
| D. | Ammonia-oxidizing bacteria (chemolithotrophic bacteria) |
| Answer» E. | |
| 27. |
An organism that expends energy to grow in a habitat with a low water activity in order to maintain internal solute concentrations to retain water is |
| A. | osmotolerant |
| B. | acidophile |
| C. | aerotolerant anaerobe |
| D. | alkalophile |
| Answer» B. acidophile | |
| 28. |
The straightforward method of binary fission explains how bacteria |
| A. | grow in nutrient agar |
| B. | evolve |
| C. | move |
| D. | reproduce |
| Answer» E. | |
| 29. |
Lag phase is also known as |
| A. | period of initial adjustment |
| B. | transitional period |
| C. | generation time |
| D. | none of these |
| Answer» B. transitional period | |
| 30. |
Which of the following is used to grow bacterial cultures continuously? |
| A. | Chemostat |
| B. | Coulter Counter |
| C. | Hemostat |
| D. | Petroff-Hausser chamber |
| Answer» B. Coulter Counter | |
| 31. |
A microbe, which grows at temperatures above 95 C is most likely to be |
| A. | an archaean |
| B. | a fungus |
| C. | a protozoan |
| D. | none of these |
| Answer» B. a fungus | |
| 32. |
Some organisms can use reduced inorganic compounds as electron donors and are termed as |
| A. | lithotrophs |
| B. | phototrophs |
| C. | chemotrophs |
| D. | photo-organotrophs |
| Answer» B. phototrophs | |
| 33. |
The organism which grows best above 45 C are called |
| A. | psychrophilic |
| B. | mesosphilic |
| C. | thermophilic |
| D. | any of these |
| Answer» D. any of these | |
| 34. |
The organism which obtain their energy from chemicals are designated as |
| A. | prototrophs |
| B. | chemotrophs |
| C. | organotrophs |
| D. | autotrophs |
| Answer» C. organotrophs | |
| 35. |
Nutrient content and biological structures are considered as |
| A. | implicit factor for microbial growth |
| B. | intrinsic factor for microbial growth |
| C. | processing factor |
| D. | none of the above |
| Answer» C. processing factor | |
| 36. |
Postgates assay technique is useful in determining if a cell is viable even though it is |
| A. | incapable of cell division |
| B. | too small to see |
| C. | an obligate aerobe |
| D. | a thermophile |
| Answer» B. too small to see | |
| 37. |
What are the extrinsic factors for the microbial growth? |
| A. | humidity |
| B. | storage temperature |
| C. | composition of gas phase |
| D. | all of these |
| Answer» E. | |
| 38. |
The combination of low levels of NaCl, NaNO3 (sodium nitrate), and slightly acid pH can prevent multiplication and toxin formation of |
| A. | <i>Salmonella</i> |
| B. | <i>S. aureus</i> |
| C. | <i>C. botulinum</i> |
| D. | all of these |
| Answer» E. | |
| 39. |
A culture broth tube was very turbid at the bottom of the tube but clear at the top of the tube indicating that the |
| A. | broth is sterile |
| B. | organism can tolerate oxygen |
| C. | organism cannot produce superoxide dismutase and/or catalase |
| D. | organism should be grown in an anaerobic chamber |
| Answer» D. organism should be grown in an anaerobic chamber | |
| 40. |
In the exponential phase, the cells and cell mass |
| A. | first increases then decreases |
| B. | decreases |
| C. | are constant |
| D. | double at a constant rate |
| Answer» E. | |
| 41. |
An organism is completely dependent on atmospheric O2 for growth. This organism is |
| A. | osmotolerant |
| B. | acidophile |
| C. | facultative anaerobe |
| D. | obligate aerobe |
| Answer» E. | |
| 42. |
The term obligate anaerobe refers to an organism that |
| A. | doesn't use oxygen but tolerates it |
| B. | is killed by oxygen |
| C. | uses oxygen when present or grows without oxygen when oxygen is absent |
| D. | prefers to grow without oxygen |
| Answer» C. uses oxygen when present or grows without oxygen when oxygen is absent | |
| 43. |
All organisms require at least small amounts of carbondioxide, However, some can use CO2 as their sole source of carbon. Such organisms are termed as |
| A. | autotrophs |
| B. | phototrophs |
| C. | chemotrophs |
| D. | photo-organotrophs |
| Answer» B. phototrophs | |
| 44. |
The generation time of a culture that produces two generations per hour is |
| A. | greater than that produces three generations per hour |
| B. | lesser than that produces three generations per hour |
| C. | equal to that produces three generations per hour |
| D. | none of the above |
| Answer» B. lesser than that produces three generations per hour | |
| 45. |
Autotrophic bacteria are those which |
| A. | make their own food |
| B. | form a long chain glycocalyx |
| C. | are highly susceptible to penicillin |
| D. | produce a blue-green pigment |
| Answer» B. form a long chain glycocalyx | |
| 46. |
Which of the following organisms typically get their carbon for biosynthesis from organic compounds? |
| A. | Aerobic, glucose-respiring bacteria (aerobic respiration) |
| B. | Ammonia-oxidizing bacteria (chemolithotrophic bacteria) |
| C. | Photosynthetic cyanobacteria (phototrophic metabolism) |
| D. | None of the above |
| Answer» B. Ammonia-oxidizing bacteria (chemolithotrophic bacteria) | |
| 47. |
The period between inoculation of bacteria in a culture medium and beginning of multiplication is known as |
| A. | stationary phase |
| B. | log phase |
| C. | lag phase |
| D. | decline phase |
| Answer» D. decline phase | |
| 48. |
The term aerotolerant anaerobe refers to an organism that |
| A. | doesn't use oxygen but can grow in the presence of oxygen |
| B. | is killed by oxygen |
| C. | requires less oxygen than is present in air |
| D. | prefers to grow without oxygen |
| Answer» B. is killed by oxygen | |
| 49. |
The equation used to obtain bacterial population by binary fission is |
| A. | N = No2 |
| B. | <sup>n</sup> |
| C. | log N/No = (0.3010) n |
| D. | log(N/No) = nlog 2 |
| E. | All of these |
| Answer» E. All of these | |
| 50. |
Organisms such as lactobacilli that have elaborate requirements for specific nutrients i.e. vitamins and other growth promoting substances, are generally called as |
| A. | fastidious hetrotrophs |
| B. | chemo-lithotrophs |
| C. | chemotrophs |
| D. | photo-organotrophs |
| Answer» B. chemo-lithotrophs | |