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				This section includes 60 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | Generation time of is | 
| A. | 20 minutes | 
| B. | 20 hours | 
| C. | 20 days | 
| D. | 200 hours | 
| Answer» B. 20 hours | |
| 2. | The total biomass of an organism will be determined by the nutrient present in the lowest concentration relative to the organism's requirements is a statement of | 
| A. | Liebig's law of the minimum | 
| B. | Shelford's law of tolerance | 
| C. | quorum sensing | 
| D. | Heisenberg's principle of uncertainty | 
| Answer» B. Shelford's law of tolerance | |
| 3. | All organisms require at least small amounts of carbondioxide, However, some can use CO as their sole source of carbon. Such organisms are termed as | 
| A. | autotrophs | 
| B. | phototrophs | 
| C. | chemotrophs | 
| D. | photo-organotrophs | 
| Answer» B. phototrophs | |
| 4. | The ability of to produce bioluminescence chemicals only when a certain population density has been reached is an example of | 
| A. | Liebig's law of the minimum | 
| B. | Shelford's law of tolerance | 
| C. | quorum sensing | 
| D. | Heisenberg's principle of uncertainty | 
| Answer» D. Heisenberg's principle of uncertainty | |
| 5. | can grow as | 
| A. | chemo-lithotrophs | 
| B. | chemo-organotrophs | 
| C. | both (a) and (b) | 
| D. | none of these | 
| Answer» D. none of these | |
| 6. | The combination of low levels of NaCl, NaNO (sodium nitrate), and slightly acid pH can prevent multiplication and toxin formation of | 
| A. | all of these | 
| Answer» E. | |
| 7. | An organism is completely dependent on atmospheric O for growth. This organism is | 
| A. | osmotolerant | 
| B. | acidophile | 
| C. | facultative anaerobe | 
| D. | obligate aerobe | 
| Answer» E. | |
| 8. | Bacteria of genus use __________ as their electron source. | 
| A. | ammonia | 
| B. | H | 
| C. | S | 
| D. | succinate | 
| E. | light | 
| Answer» B. H | |
| 9. | Which of the following is not the characteristic of a growth curve? | 
| A. | Shows development of microbial population under relatively stable environmental conditions | 
| B. | Plotted with logarithmic numbers | 
| C. | Graphs numbers of microbes versus time | 
| D. | Each growth curve consists of four distinct phases | 
| Answer» E. | |
| 10. | The term facultative anaerobe refers to an organism that | 
| A. | doesn't use oxygen but tolerates it | 
| B. | is killed by oxygen | 
| C. | uses oxygen when present or grows without oxygen when absent | 
| D. | requires less oxygen than is present in air | 
| Answer» D. requires less oxygen than is present in air | |
| 11. | Generation time of Escherichia coli is | 
| A. | 20 minutes | 
| B. | 20 hours | 
| C. | 20 days | 
| D. | 200 hours | 
| Answer» B. 20 hours | |
| 12. | Generation time is | 
| A. | time required for the population to double | 
| B. | time required for the initial adjustment | 
| C. | obtained by expression t/n, where t = time interval, n = number of generation | 
| D. | both (a) and (c) | 
| Answer» E. | |
| 13. | The microorganisms that grow best in a low-oxygen environment is called a (n) | 
| A. | aerobe | 
| B. | anaerobe | 
| C. | facultative | 
| D. | microaerophile | 
| Answer» E. | |
| 14. | Pseudomonas pseudoflava can grow as | 
| A. | chemo-lithotrophs | 
| B. | chemo-organotrophs | 
| C. | both (a) and (b) | 
| D. | none of these | 
| Answer» D. none of these | |
| 15. | Which of the following has more tolerance for acidic pH (lower pH)? | 
| A. | Yeast and moulds | 
| B. | Bacteria | 
| C. | <i>E. coli</i> | 
| D. | None of these | 
| Answer» B. Bacteria | |
| 16. | Antimicrobial constituents for the microbial growth in foods are | 
| A. | intrinsic factor | 
| B. | extrinsic factor | 
| C. | both (a) and (b) | 
| D. | none of these | 
| Answer» B. extrinsic factor | |
| 17. | The average size of cells during the exponential phase of growth is | 
| A. | greater than the lag phase | 
| B. | lesser than the lag phase | 
| C. | equal to the lag phase | 
| D. | none of these | 
| Answer» C. equal to the lag phase | |
| 18. | A culture broth tube was very turbid at the surface but clear throughout the rest of the tube indicating that the | 
| A. | organism are aerobes | 
| B. | organism should be grown in an anaerobic chamber | 
| C. | organism cannot produce superoxide dismutase and/or catalase | 
| D. | organism cannot tolerate oxygen | 
| Answer» B. organism should be grown in an anaerobic chamber | |
| 19. | The reproduction by budding occur in | 
| A. | <i>Rhodopseudomonas acidophila</i> | 
| B. | <i> Hyphomicrobium vulgare</i> | 
| C. | <i>Bacillus subtilis</i> | 
| D. | Both (a) and (b) | 
| Answer» E. | |
| 20. | A spore differs from an actively replicating bacterium in that the spore | 
| A. | is produced during a process involving asymmetric division | 
| B. | is able to withstand more extreme conditions than the replicating cell | 
| C. | is metabolically inactive | 
| D. | all of the above | 
| Answer» E. | |
| 21. | Bacteria and fungi multiply best | 
| A. | below 16 C | 
| B. | between 16-38 C | 
| C. | above 38 C | 
| D. | none of these | 
| Answer» C. above 38 C | |
| 22. | An organism has an optimal growth rate when the hydrogen ion concentration is very high. This organism is | 
| A. | osmotolerant | 
| B. | acidophile | 
| C. | neutrophile | 
| D. | aerotolerant anaerobe | 
| Answer» C. neutrophile | |
| 23. | Which of the following procedures uses a photocell to measure absorbance of a culture to regulate the flow of culture media? | 
| A. | Coulter Counter | 
| B. | Hemostat | 
| C. | Petroff-Hausser chamber | 
| D. | Trubidostat | 
| Answer» E. | |
| 24. | Organisms, using organic compounds as electron donors are called | 
| A. | lithotrophs | 
| B. | phototrophs | 
| C. | chemotrophs | 
| D. | organotrophs | 
| Answer» E. | |
| 25. | The total biomass of an organism will be determined by the nutrient present in the lowest concentration relative to the organism's requirements is a statement of | 
| A. | Liebig's law of the minimum | 
| B. | Shelford's law of tolerance | 
| C. | quorum sensing | 
| D. | Heisenberg's principle of uncertainty | 
| Answer» B. Shelford's law of tolerance | |
| 26. | Which of the following organisms typically get their carbon for biosynthesis from carbon dioxide? | 
| A. | Glucose-fermenting bacteria (fermentation) | 
| B. | Anaerobic, glucose-respiring bacteria (anaerobic respiration) | 
| C. | Aerobic, glucose-respiring bacteria (aerobic respiration) | 
| D. | Ammonia-oxidizing bacteria (chemolithotrophic bacteria) | 
| Answer» E. | |
| 27. | An organism that expends energy to grow in a habitat with a low water activity in order to maintain internal solute concentrations to retain water is | 
| A. | osmotolerant | 
| B. | acidophile | 
| C. | aerotolerant anaerobe | 
| D. | alkalophile | 
| Answer» B. acidophile | |
| 28. | The straightforward method of binary fission explains how bacteria | 
| A. | grow in nutrient agar | 
| B. | evolve | 
| C. | move | 
| D. | reproduce | 
| Answer» E. | |
| 29. | Lag phase is also known as | 
| A. | period of initial adjustment | 
| B. | transitional period | 
| C. | generation time | 
| D. | none of these | 
| Answer» B. transitional period | |
| 30. | Which of the following is used to grow bacterial cultures continuously? | 
| A. | Chemostat | 
| B. | Coulter Counter | 
| C. | Hemostat | 
| D. | Petroff-Hausser chamber | 
| Answer» B. Coulter Counter | |
| 31. | A microbe, which grows at temperatures above 95 C is most likely to be | 
| A. | an archaean | 
| B. | a fungus | 
| C. | a protozoan | 
| D. | none of these | 
| Answer» B. a fungus | |
| 32. | Some organisms can use reduced inorganic compounds as electron donors and are termed as | 
| A. | lithotrophs | 
| B. | phototrophs | 
| C. | chemotrophs | 
| D. | photo-organotrophs | 
| Answer» B. phototrophs | |
| 33. | The organism which grows best above 45 C are called | 
| A. | psychrophilic | 
| B. | mesosphilic | 
| C. | thermophilic | 
| D. | any of these | 
| Answer» D. any of these | |
| 34. | The organism which obtain their energy from chemicals are designated as | 
| A. | prototrophs | 
| B. | chemotrophs | 
| C. | organotrophs | 
| D. | autotrophs | 
| Answer» C. organotrophs | |
| 35. | Nutrient content and biological structures are considered as | 
| A. | implicit factor for microbial growth | 
| B. | intrinsic factor for microbial growth | 
| C. | processing factor | 
| D. | none of the above | 
| Answer» C. processing factor | |
| 36. | Postgates assay technique is useful in determining if a cell is viable even though it is | 
| A. | incapable of cell division | 
| B. | too small to see | 
| C. | an obligate aerobe | 
| D. | a thermophile | 
| Answer» B. too small to see | |
| 37. | What are the extrinsic factors for the microbial growth? | 
| A. | humidity | 
| B. | storage temperature | 
| C. | composition of gas phase | 
| D. | all of these | 
| Answer» E. | |
| 38. | The combination of low levels of NaCl, NaNO3 (sodium nitrate), and slightly acid pH can prevent multiplication and toxin formation of | 
| A. | <i>Salmonella</i> | 
| B. | <i>S. aureus</i> | 
| C. | <i>C. botulinum</i> | 
| D. | all of these | 
| Answer» E. | |
| 39. | A culture broth tube was very turbid at the bottom of the tube but clear at the top of the tube indicating that the | 
| A. | broth is sterile | 
| B. | organism can tolerate oxygen | 
| C. | organism cannot produce superoxide dismutase and/or catalase | 
| D. | organism should be grown in an anaerobic chamber | 
| Answer» D. organism should be grown in an anaerobic chamber | |
| 40. | In the exponential phase, the cells and cell mass | 
| A. | first increases then decreases | 
| B. | decreases | 
| C. | are constant | 
| D. | double at a constant rate | 
| Answer» E. | |
| 41. | An organism is completely dependent on atmospheric O2 for growth. This organism is | 
| A. | osmotolerant | 
| B. | acidophile | 
| C. | facultative anaerobe | 
| D. | obligate aerobe | 
| Answer» E. | |
| 42. | The term obligate anaerobe refers to an organism that | 
| A. | doesn't use oxygen but tolerates it | 
| B. | is killed by oxygen | 
| C. | uses oxygen when present or grows without oxygen when oxygen is absent | 
| D. | prefers to grow without oxygen | 
| Answer» C. uses oxygen when present or grows without oxygen when oxygen is absent | |
| 43. | All organisms require at least small amounts of carbondioxide, However, some can use CO2 as their sole source of carbon. Such organisms are termed as | 
| A. | autotrophs | 
| B. | phototrophs | 
| C. | chemotrophs | 
| D. | photo-organotrophs | 
| Answer» B. phototrophs | |
| 44. | The generation time of a culture that produces two generations per hour is | 
| A. | greater than that produces three generations per hour | 
| B. | lesser than that produces three generations per hour | 
| C. | equal to that produces three generations per hour | 
| D. | none of the above | 
| Answer» B. lesser than that produces three generations per hour | |
| 45. | Autotrophic bacteria are those which | 
| A. | make their own food | 
| B. | form a long chain glycocalyx | 
| C. | are highly susceptible to penicillin | 
| D. | produce a blue-green pigment | 
| Answer» B. form a long chain glycocalyx | |
| 46. | Which of the following organisms typically get their carbon for biosynthesis from organic compounds? | 
| A. | Aerobic, glucose-respiring bacteria (aerobic respiration) | 
| B. | Ammonia-oxidizing bacteria (chemolithotrophic bacteria) | 
| C. | Photosynthetic cyanobacteria (phototrophic metabolism) | 
| D. | None of the above | 
| Answer» B. Ammonia-oxidizing bacteria (chemolithotrophic bacteria) | |
| 47. | The period between inoculation of bacteria in a culture medium and beginning of multiplication is known as | 
| A. | stationary phase | 
| B. | log phase | 
| C. | lag phase | 
| D. | decline phase | 
| Answer» D. decline phase | |
| 48. | The term aerotolerant anaerobe refers to an organism that | 
| A. | doesn't use oxygen but can grow in the presence of oxygen | 
| B. | is killed by oxygen | 
| C. | requires less oxygen than is present in air | 
| D. | prefers to grow without oxygen | 
| Answer» B. is killed by oxygen | |
| 49. | The equation used to obtain bacterial population by binary fission is | 
| A. | N = No2 | 
| B. | <sup>n</sup> | 
| C. | log N/No = (0.3010) n | 
| D. | log(N/No) = nlog 2 | 
| E. | All of these | 
| Answer» E. All of these | |
| 50. | Organisms such as lactobacilli that have elaborate requirements for specific nutrients i.e. vitamins and other growth promoting substances, are generally called as | 
| A. | fastidious hetrotrophs | 
| B. | chemo-lithotrophs | 
| C. | chemotrophs | 
| D. | photo-organotrophs | 
| Answer» B. chemo-lithotrophs | |