MCQOPTIONS
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MCQOPTIONS
This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The position of band during electrophoresis indicates the dNTP used as a chain terminator in that mixture. |
| A. | True |
| B. | False |
| Answer» C. | |
| 2. |
Strand separation for DNA sequencing is done to ____________ |
| A. | sort the fragments according to their size |
| B. | sort the fragments according to their molecular weight |
| C. | obtain consecutive sequence as per termination |
| D. | obtain specific lanes of sequencing |
| Answer» D. obtain specific lanes of sequencing | |
| 3. |
The four reaction mixtures that were prepared, contained ____________ |
| A. | dCTP, dTTP, dGTP, dATP |
| B. | dCTP, ddTTP, dGTP, ddATP |
| C. | ddCTP, dTTP, ddGTP, dATP |
| D. | dCTP, ddTTP, dGTP, dATP |
| Answer» E. | |
| 4. |
With respect to Sanger s enzymatic method of sequencing pick the odd one out. |
| A. | Radioactive dideoxyribonucleotides |
| B. | Primers |
| C. | Klenow fragment |
| D. | Restriction digestion |
| Answer» E. | |
| 5. |
Which enzyme is used for the replication in case of Sanger s method of sequencing? |
| A. | Polymerase I |
| B. | Smaller subunit polymerase I |
| C. | Polymerase III |
| D. | Larger subunit polymerase I |
| Answer» E. | |
| 6. |
The dideoxy method is also known as _____________ |
| A. | Maxem and Gilbert method |
| B. | Autosequencing |
| C. | Sanger s enzymatic sequencing |
| D. | Pyrosequencing |
| Answer» D. Pyrosequencing | |
| 7. |
The process of electrophoresis is the key to sequencing. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
Which step is not involved in base-specific cleavage of DNA fragment? |
| A. | Modification of concerned base |
| B. | Removal of modified base from DNA strand |
| C. | Induction of random strand break |
| D. | End labeled DNA fragments of variable lengths produced |
| Answer» D. End labeled DNA fragments of variable lengths produced | |
| 9. |
What are the basic base-specific cleavage sites used in Maxem and Gilbert method? |
| A. | A, T, G, C |
| B. | C, T, A+G, T+C |
| C. | A, G, A+T, G+C |
| D. | G, C, A+G, C+T |
| Answer» E. | |
| 10. |
The 32P is added at the 3 end by polynucleotidyl kinase. |
| A. | True |
| B. | False |
| Answer» C. | |
| 11. |
End labeled DNA sequencing is known as dideoxy method of sequencing. |
| A. | True |
| B. | False |
| Answer» C. | |