MCQOPTIONS
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MCQOPTIONS
This section includes 6 Mcqs, each offering curated multiple-choice questions to sharpen your Enzyme Technology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following techniques may not be used to produce thermophilic enzymes? |
| A. | Internal salt link |
| B. | Extra internal hydrophobic residues |
| C. | Suitable exterior substitutions |
| D. | Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme |
| Answer» E. | |
| 2. |
Which of the following is not a step involved in site directed mutagenesis using a hypothetical example? |
| A. | Derive the primary structure of the enzyme from the DNA sequence |
| B. | Identification of mutant DNA by hybridization with radioactively labelled oligonucleotides |
| C. | Cleavage by Hind III |
| D. | Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme |
| Answer» D. Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme | |
| 3. |
Protein engineering directed at subtilisin has been aimed to improve the activity in detergents by stabilizing it at even higher temperatures, pH and oxidant strength with some concerned alterations. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
Which of the following is not a step in producing penicillin -G-amidase? |
| A. | Cleavage by Hind III |
| B. | Replacement of asparagine residue with serine present in the native enzyme |
| C. | Cleaved fragments cloned into a cosmid vector |
| D. | Penicillin-G-amidase gene transferred on to pBR322 plasmids |
| Answer» C. Cleaved fragments cloned into a cosmid vector | |
| 5. |
The property of increasing the amount of the enzyme by increasing the number of gene copies is used increase the activity of __________ in E. Coli. |
| A. | Subtilisin |
| B. | Trypsin |
| C. | Penicillin-G-amidase |
| D. | Glucose isomerase |
| Answer» D. Glucose isomerase | |
| 6. |
Which of the following property may not be improved or altered using genetic engineering? |
| A. | Yield and Kinetics |
| B. | Various safety aspects |
| C. | Ease of downstream processing |
| D. | Accurate, precise, reproducible and linear |
| Answer» E. | |