Explore topic-wise MCQs in Molecular Biology.

This section includes 12 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.

1.

The temperature at which DNA synthesis takes place is 74˚C.

A. True
B. False
Answer» B. False
Discussion
2.

Annealing of primer is facilitated by complementary region.

A. True
B. False
Answer» B. False
Discussion
3.

PCR is a DNA amplifying in vivo method.

A. True
B. False
Answer» C.
Discussion
4.

THE_TEMPERATURE_AT_WHICH_DNA_SYNTHESIS_TAKES_PLACE_IS_74‚ÀÖ√Ñ‚ÀÖ‚ÀÇC________________?$#

A. True
B. False
Answer» B. False
Discussion
5.

At what temperature do annealing of DNA and primer takes place?

A. 42Àö
B. 54Àö
C. 74Àö
D. 96Àö
Answer» E.
Discussion
6.

At what temperature do denaturation of DNA double helix takes place?

A. 60Àö
B. 54Àö
C. 74Àö
D. 94Àö
Answer» E.
Discussion
7.

How many DNA duplex is obtained from one DNA duplex after 4 cycles of PCR?

A. 4
B. 8
C. 16
D. 32
Answer» D. 32
Discussion
8.

Polymerase used for PCR is extracted from _____________

A. Escherichia coli
B. Homo sapiens
C. Thermus aquaticus
D. Saccharomyces cerevisiae
Answer» D. Saccharomyces cerevisiae
Discussion
9.

Primer used for the process of polymerase chain reaction are ______________

A. Single stranded DNA oligonucleotide
B. Double stranded DNA oligonucleotide
C. Single stranded RNA oligonucleotide
D. Double stranded RNA oligonucleotide
Answer» B. Double stranded DNA oligonucleotide
Discussion
10.

Annealing of primer is facilitated by complementary region ___________

A. True
B. False
Answer» B. False
Discussion
11.

Which of the following is a mismatch?

A. Polymerase – Taq polymerase
B. Template – double stranded DNA
C. Primer – oligonucleotide
D. Synthesis – 5’ to 3’ direction
Answer» C. Primer ‚Äö√Ñ√∂‚àö√ë‚àö¬® oligonucleotide
Discussion
12.

PCR is a DNA amplifying in vivo method ______

A. True
B. False
Answer» C.
Discussion