MCQOPTIONS
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MCQOPTIONS
This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Enzyme Technology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Ethanol can be removed from protein precipitate by __________ |
| A. | lyophilization |
| B. | ultrasonication |
| C. | homogenization |
| D. | dialysis |
| Answer» B. ultrasonication | |
| 2. |
What is the next step after organic solvent fractionation with dry ice ethanol water mixture? |
| A. | Refrigerated centrifugation at 3000 g for 15 mins |
| B. | Drying in freezer |
| C. | Supernatant fractionation with ethanol |
| D. | Precipitated protein dissolved in 0.01 to 0.05 M cold buffer |
| Answer» B. Drying in freezer | |
| 3. |
A preferable technique to perform fractionation is to cool the enzyme solution. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
Which of the following is a disadvantage of organic solvent fractionation? |
| A. | Faster than column techniques |
| B. | Denaturation of proteins |
| C. | Low density than salt solutions |
| D. | Short centrifugation times |
| Answer» C. Low density than salt solutions | |
| 5. |
His-Tag allows the purification to be done in fewer steps. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 6. |
Purifications of purified proteins involved in small scale lab productions meant mainly for research purposes are referred to as _____________ |
| A. | preparative purifications |
| B. | chromatographic techniques |
| C. | analytical purifications |
| D. | dialysis techniques |
| Answer» D. dialysis techniques | |
| 7. |
Insulin is purified under ___________ purifications. |
| A. | analytical |
| B. | chromatography |
| C. | preparative |
| D. | ultrafiltration |
| Answer» D. ultrafiltration | |
| 8. |
Which of the following is not a step involved in purification? |
| A. | Precipitation |
| B. | Dialysis or ultrafiltration |
| C. | Chromatographic techniques |
| D. | Freezing technique |
| Answer» E. | |