 
			 
			MCQOPTIONS
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				This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Enzyme Technology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | Which of the functional groups are not present in cation exchangers? | 
| A. | SO<sub>3</sub><sup> </sup> | 
| B. | OPO<sub>3</sub><sup> </sup> | 
| C. | NR<sub>3</sub><sup>+</sup> | 
| D. | COO<sup> </sup> | 
| Answer» D. COO<sup> </sup> | |
| 2. | Cation exchange resin is used to purify __________ | 
| A. | alkaline protease | 
| B. | peroxidase | 
| C. | alpha amylase | 
| D. | cytochrome c | 
| Answer» E. | |
| 3. | Alkaline protease is purified by using ________ | 
| A. | DEAE-cellulose | 
| B. | Q-sepharose | 
| C. | Amberlite CG-50 | 
| D. | Sephacryl S-300 | 
| Answer» B. Q-sepharose | |
| 4. | Peroxidases can be purified using ___________ | 
| A. | gel filtration | 
| B. | cation exchanger | 
| C. | anion exchanger | 
| D. | affinity chromatography | 
| Answer» D. affinity chromatography | |
| 5. | Which of these is not an anion exchanger? | 
| A. | Diethylaminoethyl (DEAE) | 
| B. | Quaternary aminoethyl (QAE) | 
| C. | Sulphopropyl (SP) | 
| D. | Quaternary ammonium (Q) | 
| Answer» D. Quaternary ammonium (Q) | |
| 6. | Cation exchange chromatography retains positively charged cations. | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 7. | When pH of enzyme solution is below their isoelectric point, the enzymes will be negatively charged and bind to anion exchangers. | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 8. | _____________ retains analyte molecules based on the coulombic interaction. | 
| A. | Thin layer chromatography | 
| B. | Affinity chromatography | 
| C. | Gel filtration | 
| D. | Ion exchange chromatography | 
| Answer» E. | |