MCQOPTIONS
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MCQOPTIONS
This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Enzyme Technology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
In _________ elution, the compounds are eluted by increasing the strength of the organic solvent during HPLC. |
| A. | nonspecific |
| B. | gradient |
| C. | specific |
| D. | isocratic |
| Answer» C. specific | |
| 2. |
In HPLC, the compounds are eluted using a constant mobile phase composition and is referred to as _____________ |
| A. | nonspecific elution |
| B. | isocratic elution |
| C. | specific elution |
| D. | gradient elution |
| Answer» C. specific elution | |
| 3. |
The gradient separates the analyte mixture as a function of affinity of the analyte for the mobile phase relative to stationary phase. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
To alter the retention time, which of the following parameters need not be manipulated? |
| A. | Choice of column |
| B. | Choice of mobile phase |
| C. | Choice in flow rate |
| D. | Sample type |
| Answer» E. | |
| 5. |
The process of determining the unknown concentration of a compound in a known solution by using HPLC is referred to as _______________ |
| A. | quantification of a compound |
| B. | purification of a compound |
| C. | chemical separations |
| D. | identification of a compound |
| Answer» B. purification of a compound | |
| 6. |
__________________ refers to the process of separating or extracting the target compound from other compounds or contaminants. |
| A. | Quantification of a compound |
| B. | Identification of a compound |
| C. | Purification of a compound |
| D. | Chemical separations |
| Answer» D. Chemical separations | |
| 7. |
_____________ can be achieved using HPLC by utilizing the fact that certain compounds have different migration rates given a particular column and mobile phase. |
| A. | Purification of a compound |
| B. | Chemical separations |
| C. | Identification of a compound |
| D. | Quantification of compound |
| Answer» C. Identification of a compound | |
| 8. |
Which of the following information cannot be obtained by using analytical HPLC? |
| A. | Isolation of a compound |
| B. | Identification of a compound |
| C. | Quantification of a compound |
| D. | Resolution of a compound |
| Answer» B. Identification of a compound | |
| 9. |
_______________ refers to process of isolation and purification of compounds. |
| A. | FPLC |
| B. | Analytical HPLC |
| C. | Preparative HPLC |
| D. | TLC |
| Answer» D. TLC | |
| 10. |
The time at which specific analyte elute out is termed as _________ |
| A. | K<sub>m</sub> |
| B. | half-life |
| C. | retention time |
| D. | pK<sub>a</sub> |
| Answer» D. pK<sub>a</sub> | |
| 11. |
The chromatography in which the sample is forced through a column of the stationary phase by pumping a liquid (mobile phase) at high pressure through the column is referred to as ___________ |
| A. | affinity chromatography |
| B. | fast protein liquid chromatography (FPLC) |
| C. | thin layer chromatography (TLC) |
| D. | high performance liquid chromatography (HPLC) |
| Answer» E. | |