MCQOPTIONS
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MCQOPTIONS
This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Enzyme Technology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Acid phosphatase: Mannose:: Alpha amylase: ______________ |
| A. | KCl |
| B. | Glycogen |
| C. | Adenosine |
| D. | Methyl-D-mannoside |
| Answer» C. Adenosine | |
| 2. |
Acetylcholine esterase can be purified by using affinity chromatography with the use of _______ as a ligand. |
| A. | ATP |
| B. | NADP |
| C. | Acrdinium |
| D. | Con A |
| Answer» D. Con A | |
| 3. |
Which among these is not a way of non-specific elution? |
| A. | Solvent change |
| B. | pH change |
| C. | Reversible denaturation |
| D. | Competition to suitable complementary protein |
| Answer» E. | |
| 4. |
__________ refers to eluting a compound by creating a competition between the bounded enzymes with its suitable complimentary protein in solution. |
| A. | Specific elution |
| B. | Nonspecific elution |
| C. | Filtration |
| D. | Dialysis |
| Answer» B. Nonspecific elution | |
| 5. |
The first step in preparation of affinity chromatography column is _____________ |
| A. | ligand attachment to matrix |
| B. | coupling of aromatic amines to matrix |
| C. | activation process |
| D. | precipitation |
| Answer» D. precipitation | |
| 6. |
The matrix selected during affinity chromatography must possess certain chemical groups. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 7. |
In affinity chromatography, the matrix should be chemically and mechanically stable. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
Which of this is not true while selecting a solid matrix or column during affinity chromatography? |
| A. | The matrix should interact weakly with enzymes |
| B. | The matrix should be based on inorganic compounds |
| C. | The matrix should exhibit good flow property |
| D. | The matrix should be highly porous |
| Answer» C. The matrix should exhibit good flow property | |
| 9. |
Which of the following is not a highly specific biological interaction to be used in affinity chromatography? |
| A. | Cations-anions |
| B. | Antigen-antibody |
| C. | Enzyme-substrate |
| D. | Receptor-ligand |
| Answer» B. Antigen-antibody | |
| 10. |
The chromatographic method of separating biochemical mixture of compounds, based on highly specific biological interactions is referred to as ______________ |
| A. | thin layer chromatography |
| B. | ion-exchange chromatography |
| C. | affinity chromatography |
| D. | gel permeation chromatography |
| Answer» D. gel permeation chromatography | |