Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are

free α-, β- or γ carb oxyl groups

phenyl, hydroxyl, sulfhydryl or imidazole groups

To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as

rate if not slowed by diffusion/ actual reaction rate

reaction rate/rate if not slowed by diffusion

rate if not slowed by diffusion/ reaction rate

actual reaction rate/ rate if not slowed by diffusion

rate if not slowed by diffusion/ actual reaction rate

The immobilized enzyme produced by micro encapsulation technique provides

an extremely large surface area

relatively smaller surface area

The immobilized technique involving chemical method is

non-covalent bond formation dependent

covalent bond formation dependent

non-covalent bond formation dependent

Damkohler number(NDa) is

the ratio of the minimum reaction rate to the maximum mass transfer rate

the ratio of the maximum reaction rate to the maximum mass transfer rate

the ratio of the minimum reaction rate to the maximum mass transfer rate

the ratio of the maximum reaction rate to the minimum mass transfer rate

the ratio of the minimum reaction rate to the minimum mass transfer rate

The immobilization technique involving physical method is

covalent bond formation dependent

non-covalent bond formation dependent

Which of the following is considered as a disadvantage to the adsorption method of immobilization?

It is possible to separate and purify the enzymes while being immobilized

The enzymes are not usually deactivated by adsorption

The adsorption is a reversible process

State of immobilization is very sensitive to solution pH, ionic strength and temperature

Which is not the method for producing immobilized enzymes with multifunctional reagents?

Enzymes are adsorbed on the surface active support followed by intermolecular cross linking

Functional groups are introduced on the support to react co-valently with enzymes

Enzymes are cross linked intermolecularly

The covalent attachment of enzyme molecules is via

essential amino acids residues to water insoluble, functional supports

nonessential amino acids residues to water insoluble, functional supports

essential amino acids residues to water insoluble, functional supports

nonessential amino acids residues to water soluble, functional supports

essential amino acids residues to water soluble, functional supports

Which of the following is taken as an assumption in the distributed model?

The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.

Mass transfer through the immobilized enzyme occurs via molecular diffusion

There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme

The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by

crosslinking using multifunctional reagents

copolymerization or microencapsulation

crosslinking using multifunctional reagents

The effectiveness factor increases with the

increase of diffusivity and increased with the increase of particle size

increase of diffusivity and decreased with the increase of particle size

increase of diffusivity and increased with the increase of particle size

decrease of diffusivity and decreased with the increase of particle size

increase of diffusivity and decrease with the decrease of particle size

Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses

a diffusion cell with two compartments

a diffusion cell with two compartments divided by a thin gel

a diffusion cell with two compartments

a diffusion cell with two compartments plus by a thin gel

a diffusion cell with two compartments multiply by a thin gel

During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is

more than that of substrate consumption

equal to that of substrate consumption

more than that of substrate consumption

lesser than that of substrate consumption

is nothing to do with the substrate consumption

Which of the following is not the correct answer?

Immobilized enzyme may show selectively altered chemical properties

Immobilized enzyme may show selectively altered physical properties

Immobilized enzyme may simulate the realistic natural environment where the enzyme come from the cell

The given graph shows the effect of substrate concentration on the rate of reaction of the enzyme green gram-phosphatase What does the graph indicate [AIIMS 2005]

Formation of an enzyme-substrate complex

The rate of enzyme reaction is directly proportional to the substrate concentration

Presence of an enzyme inhibitor in the reaction mixture

Formation of an enzyme-substrate complex

At higher substrate concentration the pH increase

Assertion : The higher the turn-over number the more efficient an enzyme is. Reason : It is not dependent upon the number of active sites present over an enzyme.

If both the assertion and reason are false

If both the assertion and the reason are true and the reason is a correct explanation of the assertion

If both the assertion and reason are true but the reason is not a correct explanation of the assertion

If the assertion is true but the reason is false

If both the assertion and reason are false

If the assertion is false but reason is true

36 + Mcqs in Enzyme in Biology Page 1 McqOptions

1.	Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are
A.	free α-, β- or γ carb oxyl groups
B.	α or β amino groups
C.	phenyl, hydroxyl, sulfhydryl or imidazole groups
D.	all of the above
Answer» E.

Discussion

2.	To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as
A.	reaction rate/rate if not slowed by diffusion
B.	rate if not slowed by diffusion/ reaction rate
C.	actual reaction rate/ rate if not slowed by diffusion
D.	rate if not slowed by diffusion/ actual reaction rate
Answer» D. rate if not slowed by diffusion/ actual reaction rate

Discussion

3.	The immobilized enzyme produced by micro encapsulation technique provides
A.	an extremely large surface area
B.	smaller surface area
C.	high amount of solvent
D.	relatively smaller surface area
Answer» B. smaller surface area

Discussion

4.	The immobilized technique involving chemical method is
A.	covalent bond formation dependent
B.	non-covalent bond formation dependent
C.	both (a) and (b)
D.	ionic bond formation dependent
Answer» B. non-covalent bond formation dependent

Discussion

5.	Which of the following is the commonly employed adsorbents?
A.	Calcium carbonate
B.	Alumina
C.	Celluloses
D.	All of these
Answer» E.

Discussion

6.	The support material for immobilization of cells of Bacillus subtilis is
A.	ion exchange resins
B.	gelatin
C.	Anthracite
D.	agarose and carbodiimide
Answer» E.

Discussion

7.	For a steady state condition, the change of substrate concentration (dCs/dt) is
A.	zero
B.	1
C.	>1
D.	<1
Answer» B. 1

Discussion

8.	Which medium is used for the production of Penicillin using immobilized cells
A.	1% peptone medium
B.	glucose medium
C.	Yeast extract medium
D.	LB broth
Answer» C. Yeast extract medium

Discussion

9.	Which of the following is not a physical method of immobilization?
A.	Adsorption
B.	Entrapment
C.	Micro encapsulation
D.	None of these
Answer» E.

Discussion

10.	Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have
A.	low molecular weight
B.	high molecular weight
C.	high equivalent weight
D.	low reactivity
Answer» B. high molecular weight

Discussion

11.	Damkohler number(NDa) is
A.	the ratio of the maximum reaction rate to the maximum mass transfer rate
B.	the ratio of the minimum reaction rate to the maximum mass transfer rate
C.	the ratio of the maximum reaction rate to the minimum mass transfer rate
D.	the ratio of the minimum reaction rate to the minimum mass transfer rate
Answer» B. the ratio of the minimum reaction rate to the maximum mass transfer rate

Discussion

12.	The immobilization technique involving physical method is
A.	covalent bond formation dependent
B.	non-covalent bond formation dependent
C.	both (a) and (b)
D.	ionic bond formation dependent
Answer» C. both (a) and (b)

Discussion

13.	Which of the following is considered as a disadvantage to the adsorption method of immobilization?
A.	It is possible to separate and purify the enzymes while being immobilized
B.	The enzymes are not usually deactivated by adsorption
C.	The adsorption is a reversible process
D.	State of immobilization is very sensitive to solution pH, ionic strength and temperature
Answer» E.

Discussion

14.	Which is not the method for producing immobilized enzymes with multifunctional reagents?
A.	Enzymes are adsorbed on the surface active support followed by intermolecular cross linking
B.	Functional groups are introduced on the support to react co-valently with enzymes
C.	Enzymes are cross linked intermolecularly
D.	None of the above
Answer» E.

Discussion

15.	The covalent attachment of enzyme molecules is via
A.	nonessential amino acids residues to water insoluble, functional supports
B.	essential amino acids residues to water insoluble, functional supports
C.	nonessential amino acids residues to water soluble, functional supports
D.	essential amino acids residues to water soluble, functional supports
Answer» B. essential amino acids residues to water insoluble, functional supports

Discussion

16.	Which of the following is taken as an assumption in the distributed model?
A.	The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.
B.	Mass transfer through the immobilized enzyme occurs via molecular diffusion
C.	There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme
D.	All of the above
Answer» E.

Discussion

17.	The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by
A.	copolymerization or microencapsulation
B.	crosslinking using multifunctional reagents
C.	adsorption
D.	all of the above
Answer» B. crosslinking using multifunctional reagents

Discussion

18.	The effectiveness factor increases with the
A.	increase of diffusivity and decreased with the increase of particle size
B.	increase of diffusivity and increased with the increase of particle size
C.	decrease of diffusivity and decreased with the increase of particle size
D.	increase of diffusivity and decrease with the decrease of particle size
Answer» B. increase of diffusivity and increased with the increase of particle size

Discussion

19.	Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses
A.	a diffusion cell with two compartments divided by a thin gel
B.	a diffusion cell with two compartments
C.	a diffusion cell with two compartments plus by a thin gel
D.	a diffusion cell with two compartments multiply by a thin gel
Answer» B. a diffusion cell with two compartments

Discussion

20.	During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is
A.	equal to that of substrate consumption
B.	more than that of substrate consumption
C.	lesser than that of substrate consumption
D.	is nothing to do with the substrate consumption
Answer» B. more than that of substrate consumption

Discussion

21.	Which of the following is not the correct answer?
A.	Immobilized enzyme may show selectively altered chemical properties
B.	Immobilized enzyme may show selectively altered physical properties
C.	Immobilized enzyme may simulate the realistic natural environment where the enzyme come from the cell
D.	None of the above
Answer» E.

Discussion

22.	Commonly employed water insoluble supports for the covalent attachment of enzyme include
A.	acrylamide based polymers
B.	polypeptides
C.	dextran
D.	all of these
Answer» E.

Discussion

23.	The most commonly employed cross-linked polymer is the
A.	polyacrylamide gel
B.	collagen
C.	celluloses
D.	cation exchange resin
Answer» B. collagen

Discussion

24.	If Damkohler number, NDa >>1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where Csb and Cs are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. ks mass transfer coefficient, and a is the surface area of an immobilized enzyme particles)
A.	rp = ksa(Csb-Cs)
B.	rp = ksaCsb
C.	rp = ksaCs
D.	rp = ksa(Csb+Cs)
Answer» C. rp = ksaCs

Discussion

25.	For glucose isomerization by immobilized enzyme, the reactor generally used is
A.	CSTR
B.	plug flow
C.	packed bed
D.	fluidized bed
Answer» D. fluidized bed

Discussion

26.	How many genes determine synthesis of one enzyme
A.	One
B.	Two
C.	Four
D.	Sixteen
Answer» B. Two

Discussion

27.	Which one of the following enzyme is composed of simple proteins
A.	Peroxidase
B.	Phosphoglyceromutase
C.	Proteinase
D.	Amylase
Answer» E.

Discussion

28.	At the time of cotton seeds germination, the stored food is digested by [CPMT 1996]
A.	Diastase
B.	Maltase
C.	Lipase
D.	Amylase
Answer» E.

Discussion

29.	The given graph shows the effect of substrate concentration on the rate of reaction of the enzyme green gram-phosphatase What does the graph indicate [AIIMS 2005]
A.	The rate of enzyme reaction is directly proportional to the substrate concentration
B.	Presence of an enzyme inhibitor in the reaction mixture
C.	Formation of an enzyme-substrate complex
D.	At higher substrate concentration the pH increase
Answer» C. Formation of an enzyme-substrate complex

Discussion

30.	In the modern system of nomenclature which one of the following enzyme occupies 1st position
A.	Oxidoreductase
B.	Transferase
C.	Hydrolase
D.	Ligase
Answer» B. Transferase

Discussion

31.	Enzymes which are slightly different in molecular structure but can perform identical activity are called [BHU 1991]
A.	Isoenzymes
B.	Holoenzymes
C.	Apoenzymes
D.	Coenzymes
Answer» B. Holoenzymes

Discussion

32.	As temperature changes from\[{{3}^{o}}C\]to\[{{45}^{o}}C\], the rate of enzyme activity will [MP PMT 1996]
A.	Decrease and then increase
B.	Increase and then decrease
C.	Increase only
D.	Decrease only
Answer» C. Increase only

Discussion

33.	The enzyme which converts proteins into peptones is [CPMT 1983]
A.	Erypsin
B.	Pepsin
C.	Trypsin
D.	Lipase
Answer» C. Trypsin

Discussion

34.	Which enzyme digests the stored food material of castor seeds [DPMT 1985]
A.	Lipase
B.	Amylase
C.	Diastase
D.	Protease
Answer» B. Amylase

Discussion

35.	Term - ase is used for
A.	Classification of enzymes
B.	Enzymes with special function
C.	Nomenclature of enzymes
D.	All the above
Answer» D. All the above

Discussion

36.	Assertion : The higher the turn-over number the more efficient an enzyme is. Reason : It is not dependent upon the number of active sites present over an enzyme.
A.	If both the assertion and the reason are true and the reason is a correct explanation of the assertion
B.	If both the assertion and reason are true but the reason is not a correct explanation of the assertion
C.	If the assertion is true but the reason is false
D.	If both the assertion and reason are false
E.	If the assertion is false but reason is true
Answer» D. If both the assertion and reason are false

Discussion

Explore topic-wise MCQs in Biology.

Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are

To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as

The immobilized enzyme produced by micro encapsulation technique provides

The immobilized technique involving chemical method is

Which of the following is the commonly employed adsorbents?

The support material for immobilization of cells of Bacillus subtilis is

For a steady state condition, the change of substrate concentration (dCs/dt) is

Which medium is used for the production of Penicillin using immobilized cells

Which of the following is not a physical method of immobilization?

Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have

Damkohler number(NDa) is

The immobilization technique involving physical method is

Which of the following is considered as a disadvantage to the adsorption method of immobilization?

Which is not the method for producing immobilized enzymes with multifunctional reagents?

The covalent attachment of enzyme molecules is via

Which of the following is taken as an assumption in the distributed model?

The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by

The effectiveness factor increases with the

Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses

During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is

Which of the following is not the correct answer?

Commonly employed water insoluble supports for the covalent attachment of enzyme include

The most commonly employed cross-linked polymer is the

For glucose isomerization by immobilized enzyme, the reactor generally used is

How many genes determine synthesis of one enzyme

Which one of the following enzyme is composed of simple proteins

At the time of cotton seeds germination, the stored food is digested by [CPMT 1996]

The given graph shows the effect of substrate concentration on the rate of reaction of the enzyme green gram-phosphatase What does the graph indicate [AIIMS 2005]

In the modern system of nomenclature which one of the following enzyme occupies 1st position

Enzymes which are slightly different in molecular structure but can perform identical activity are called [BHU 1991]

As temperature changes from\[{{3}^{o}}C\]to\[{{45}^{o}}C\], the rate of enzyme activity will [MP PMT 1996]

The enzyme which converts proteins into peptones is [CPMT 1983]

Which enzyme digests the stored food material of castor seeds [DPMT 1985]

Term - ase is used for

Assertion : The higher the turn-over number the more efficient an enzyme is. Reason : It is not dependent upon the number of active sites present over an enzyme.