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This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Technology knowledge and support exam preparation. Choose a topic below to get started.
1. |
Which of the following enzyme is assayed and measured using radioisotope method? |
A. | Hydrolases |
B. | Bacterial luciferase |
C. | Phosphofructokinase |
D. | Glutamate decarboxylase |
Answer» E. | |
2. |
Which of the following is not true for radioisotope method? |
A. | This method is used in studying mechanism of enzyme action and in ligand binding sites |
B. | Change is absorbance is used as the basis for assaying enzyme |
C. | If one product is gas, the method is easier |
D. | Glutamate decarboxylase is assayed and measured using this method |
Answer» C. If one product is gas, the method is easier | |
3. |
Which of the following is true for radioisotope method? |
A. | The intensity of emitted light is used to study enzyme reaction |
B. | The reaction can be used to assay ATP and appropriate enzymes via coupled reaction |
C. | It is restricted to separation of radiolabeled forms of substrate and product |
D. | Bacterial luciferase can be assayed and measured by this method |
Answer» D. Bacterial luciferase can be assayed and measured by this method | |
4. |
Which of the following is not true for luminescence method? |
A. | The intensity of emitted light is used to study enzyme reaction |
B. | The reaction can be used to assay ATP and appropriate enzymes via coupled reaction |
C. | Bacterial luciferase can be assayed and measured by this method |
D. | It is based on stoke shift |
Answer» E. | |
5. |
Continuous assay: Glucose-6-phosphate dehydrogenase:: Luminescence method: ____________ |
A. | Hydrolases |
B. | Bacterial luciferase |
C. | Ornithine decarboxylase |
D. | Glutamate decarboxylase |
Answer» C. Ornithine decarboxylase | |
6. |
In which of the following methods, the intensity of emitted light is used to study enzyme reaction? |
A. | Discontinuous assay |
B. | Luminescence method |
C. | Biosensors |
D. | Spectrophotometer |
Answer» D. Spectrophotometer | |
7. |
Which of the following enzyme is assayed and measured using spectrofluorometric method? |
A. | Glucose-6-phosphate dehydrogenase |
B. | Hydrolases |
C. | Phosphofructokinase |
D. | Glutamate decarboxylase |
Answer» C. Phosphofructokinase | |
8. |
Which of the following is not true for spectrofluorometric method? |
A. | The intensity of emitted light is used to study enzyme reaction |
B. | Trace impurities in enzyme can stop the emitted radiation |
C. | It is based on stoke shift |
D. | Hydrolases can be assayed and measured by using this method |
Answer» B. Trace impurities in enzyme can stop the emitted radiation | |
9. |
The increase in wavelength due to absorption in UV region and emission in visible light is known as ____________ |
A. | enzyme activity |
B. | enzyme assay |
C. | stoke shift |
D. | autolysis |
Answer» D. autolysis | |
10. |
Which of the following enzyme is assayed and measured using Spectrophotometer? |
A. | Hydrolases |
B. | Bacterial luciferase |
C. | Phosphofructokinase |
D. | Glutamate decarboxylase |
Answer» D. Glutamate decarboxylase | |
11. |
The change in absorbance is used as the basis for assaying enzymes using __________ |
A. | radioisotope method |
B. | luminescence method |
C. | biosensors |
D. | spectrophotometer |
Answer» E. | |