 
			 
			MCQOPTIONS
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				This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Enzyme Technology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | Which of the following enzyme is assayed and measured using radioisotope method? | 
| A. | Hydrolases | 
| B. | Bacterial luciferase | 
| C. | Phosphofructokinase | 
| D. | Glutamate decarboxylase | 
| Answer» E. | |
| 2. | Which of the following is not true for radioisotope method? | 
| A. | This method is used in studying mechanism of enzyme action and in ligand binding sites | 
| B. | Change is absorbance is used as the basis for assaying enzyme | 
| C. | If one product is gas, the method is easier | 
| D. | Glutamate decarboxylase is assayed and measured using this method | 
| Answer» C. If one product is gas, the method is easier | |
| 3. | Which of the following is true for radioisotope method? | 
| A. | The intensity of emitted light is used to study enzyme reaction | 
| B. | The reaction can be used to assay ATP and appropriate enzymes via coupled reaction | 
| C. | It is restricted to separation of radiolabeled forms of substrate and product | 
| D. | Bacterial luciferase can be assayed and measured by this method | 
| Answer» D. Bacterial luciferase can be assayed and measured by this method | |
| 4. | Which of the following is not true for luminescence method? | 
| A. | The intensity of emitted light is used to study enzyme reaction | 
| B. | The reaction can be used to assay ATP and appropriate enzymes via coupled reaction | 
| C. | Bacterial luciferase can be assayed and measured by this method | 
| D. | It is based on stoke shift | 
| Answer» E. | |
| 5. | Continuous assay: Glucose-6-phosphate dehydrogenase:: Luminescence method: ____________ | 
| A. | Hydrolases | 
| B. | Bacterial luciferase | 
| C. | Ornithine decarboxylase | 
| D. | Glutamate decarboxylase | 
| Answer» C. Ornithine decarboxylase | |
| 6. | In which of the following methods, the intensity of emitted light is used to study enzyme reaction? | 
| A. | Discontinuous assay | 
| B. | Luminescence method | 
| C. | Biosensors | 
| D. | Spectrophotometer | 
| Answer» D. Spectrophotometer | |
| 7. | Which of the following enzyme is assayed and measured using spectrofluorometric method? | 
| A. | Glucose-6-phosphate dehydrogenase | 
| B. | Hydrolases | 
| C. | Phosphofructokinase | 
| D. | Glutamate decarboxylase | 
| Answer» C. Phosphofructokinase | |
| 8. | Which of the following is not true for spectrofluorometric method? | 
| A. | The intensity of emitted light is used to study enzyme reaction | 
| B. | Trace impurities in enzyme can stop the emitted radiation | 
| C. | It is based on stoke shift | 
| D. | Hydrolases can be assayed and measured by using this method | 
| Answer» B. Trace impurities in enzyme can stop the emitted radiation | |
| 9. | The increase in wavelength due to absorption in UV region and emission in visible light is known as ____________ | 
| A. | enzyme activity | 
| B. | enzyme assay | 
| C. | stoke shift | 
| D. | autolysis | 
| Answer» D. autolysis | |
| 10. | Which of the following enzyme is assayed and measured using Spectrophotometer? | 
| A. | Hydrolases | 
| B. | Bacterial luciferase | 
| C. | Phosphofructokinase | 
| D. | Glutamate decarboxylase | 
| Answer» D. Glutamate decarboxylase | |
| 11. | The change in absorbance is used as the basis for assaying enzymes using __________ | 
| A. | radioisotope method | 
| B. | luminescence method | 
| C. | biosensors | 
| D. | spectrophotometer | 
| Answer» E. | |