In an experiment you use DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.

No difference from intact DNA pol I replication

Replication will be error prone

You grow a bacterial culture in a media containing N15 and transfer it to a media with N14. After two rounds of replication you perform a CsCl density gradient centrifugation of the DNA. How many bands will you observe what will be their intensity?

Three with middle one more intense than upper and lower

Explore topic-wise MCQs in Cytogenetics Questions and Answers.

This section includes 3 Mcqs, each offering curated multiple-choice questions to sharpen your Cytogenetics Questions and Answers knowledge and support exam preparation. Choose a topic below to get started.

1.	In an experiment you use DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.
A.	No difference from intact DNA pol I replication
B.	Replication will be slower
C.	Replication will be error prone
D.	DNA produced will be shorter
Answer» E.

Discussion

2.	How many prokaryotic DNA polymerases have 5’->3’ proofreading activity?
A.	1
B.	2
C.	3
D.	4
Answer» B. 2

Discussion

3.	You grow a bacterial culture in a media containing N15 and transfer it to a media with N14. After two rounds of replication you perform a CsCl density gradient centrifugation of the DNA. How many bands will you observe what will be their intensity?
A.	One, very intense
B.	Two, equally intense
C.	Three with middle one more intense than upper and lower
D.	Three equally intense
Answer» C. Three with middle one more intense than upper and lower

Discussion

Explore topic-wise MCQs in Cytogenetics Questions and Answers.

In an experiment you use DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.

How many prokaryotic DNA polymerases have 5’->3’ proofreading activity?

You grow a bacterial culture in a media containing N15 and transfer it to a media with N14. After two rounds of replication you perform a CsCl density gradient centrifugation of the DNA. How many bands will you observe what will be their intensity?