 
			 
			MCQOPTIONS
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				This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | The gaps formed by the hydrolyzing the RNA from RNA:DNA hybrid is filled by DNA polymerase I and the nicks are ligated by T4 ligase. | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 2. | Which of the following is not used for degrading RNA from RNA:DNA hybrid in replication? | 
| A. | RNase A | 
| B. | RNase H | 
| C. | Polymerase I | 
| D. | Exonuclease | 
| Answer» B. RNase H | |
| 3. | Primer synthesis short stretches of DNA for replication. | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 4. | WHICH_OF_THE_FOLLOWING_IS_NOT_USED_FOR_DEGRADING_RNA_FROM_RNA:DNA_HYBRID_IN_REPLICATION??$ | 
| A. | RNase A | 
| B. | RNase H | 
| C. | Polymerase I | 
| D. | Exonuclease | 
| Answer» B. RNase H | |
| 5. | The_gaps_formed_by_the_hydrolyzing_the_RNA_from_RNA:DNA_hybrid_is_filled_by_DNA_polymerase_I_and_the_nicks_are_ligated_by_T4_ligase.$ | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 6. | Primer synthesis short stretches of DNA for replication? | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 7. | In the case of a circular DNA synthesis how many replication forks are observed? | 
| A. | 1 | 
| B. | 2 | 
| C. | 3 | 
| D. | 4 | 
| Answer» C. 3 | |
| 8. | Who was the first person to analyse the process of replication and on which organism? | 
| A. | Arthur Kornberg: E. coli | 
| B. | John Cairns: E. coli | 
| C. | Arthur Kornberg: Bacillus subtilis | 
| D. | John Cairns: Bacillus subtilis | 
| Answer» C. Arthur Kornberg: Bacillus subtilis | |
| 9. | Which of the following does not affect DNA replication? | 
| A. | Antiparallel nature of DNA | 
| B. | End specificity of polymerase | 
| C. | SSB protein | 
| D. | Helicase | 
| Answer» D. Helicase | |
| 10. | Replication fork is the junction between the two ___________ | 
| A. | Unreplicated DNA | 
| B. | Newly synthesized DNA | 
| C. | Newly separated DNA strands and newly synthesized DNA strands | 
| D. | Newly separated DNA strands and the unreplicated DNA | 
| Answer» E. | |
| 11. | Which of the following is correctly matched with its subsequent role? | 
| A. | Topoisomerase II- can remove both positive and negative supercoil in the DNA duplex | 
| B. | Polymerase I- larger fragment responsible for exonuclease activity | 
| C. | DnaA protein- responsible for “melting” of the DNA double helix during replication | 
| D. | DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand | 
| Answer» D. DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand | |
| 12. | Pick the correct pair with respect to primers used in DNA replication. | 
| A. | RNA primer- for prokaryotes only | 
| B. | DNA primer-for eukaryotes only | 
| C. | DNA primer- for both prokaryotes and eukaryotes | 
| D. | RNA primer- for both prokaryotes and eukaryotes | 
| Answer» E. | |
| 13. | Semiconservative nature of replication of eukaryotic chromosome was first demonstrated by _______________ | 
| A. | Walter Flemming on root tip cells of Vicia faba | 
| B. | J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Vicia faba | 
| C. | Walter Flemming on root tip cells of Phaseolus vulgaris | 
| D. | J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Phaseolus vulgaris | 
| Answer» C. Walter Flemming on root tip cells of Phaseolus vulgaris | |