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This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
1. |
Both strands of DNA serve as templates concurrently in |
A. | excision repair |
B. | replication |
C. | none of these |
D. | mismatch repair |
Answer» C. none of these | |
2. |
Which of the following enzymes unwind short stretches of DNA helix immediately ahead of a replication fork? |
A. | Helicases |
B. | DNA polymerases |
C. | Topoisomerases |
D. | Single-stranded binding proteins |
Answer» B. DNA polymerases | |
3. |
Proofreading activity to maintain the fidelity of DNA synthesis |
A. | is a function of the 3'-5' exonuclease activity of the DNA polymerases |
B. | occurs after the synthesis has been completed |
C. | occurs in prokaryotes but not eukaryotes |
D. | requires the presence of an enzyme separate from the DNA polymerases |
Answer» B. occurs after the synthesis has been completed | |
4. |
DNA replication rates in prokaryotes are approximately of the order of |
A. | 1,00 bases per second |
B. | 10 bases per second |
C. | 10,000 bases per second |
D. | 1,000 bases per second |
Answer» C. 10,000 bases per second | |
5. |
Which of the following repairs nicked DNA by forming a phosphodiester bond between adjacent nucleotides? |
A. | DNA gyrase |
B. | Helicase |
C. | DNA ligase |
D. | Topoisomerases |
Answer» D. Topoisomerases | |
6. |
During which of the following process a new copy of a DNA molecule is precisely synthesized? |
A. | Transcription |
B. | Trasformation |
C. | Replication |
D. | Translation |
Answer» D. Translation | |
7. |
The synthesis of DNA by DNA polymerase occurs in the |
A. | 5' to 5' direction |
B. | 3' to 5' direction |
C. | 3' to 3' direction |
D. | 5' to 3' direction |
Answer» E. | |
8. |
In E.coli, which enzyme synthesizes the RNA primer for Okazaki fragments? |
A. | DnaC |
B. | DnaA |
C. | all of these |
D. | DnaG |
Answer» E. | |
9. |
The enzyme that catalyzes the synthesis of DNA is called |
A. | DNA gyrase |
B. | DNA polymerase |
C. | helicase |
D. | DNA ligase |
Answer» C. helicase | |
10. |
Which of the following statements on replication in E.coli is correct? |
A. | It occurs in bidirectional manner |
B. | It occurs in a unidirectional manner |
C. | Occurs only when λ phase has infected E. coli |
D. | Always uses T7 DNA polymerase when infected by T7 phage |
Answer» B. It occurs in a unidirectional manner | |