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This section includes 9 Mcqs, each offering curated multiple-choice questions to sharpen your Bioprocess Engineering Questions and Answers knowledge and support exam preparation. Choose a topic below to get started.
1. |
1.5 μg of enzyme gives a vmax of 3 μmol product produced per minute. What is the turnover number for this enzyme? |
A. | 100 sec-1 |
B. | 200 sec-1 |
C. | 1000 sec-1 |
D. | 2000 sec-1 |
Answer» D. 2000 sec-1 | |
2. |
The concept of “induced fit” refers to the fact that _______ |
A. | When a substrate binds to an enzyme, the enzyme induces a loss of water (desolvation) from the substrate |
B. | Substrate binding may induce a conformational change in the enzyme, which then brings catalytic groups into proper orientation |
C. | Enzyme-substrate binding induces an increase in the reaction entropy, thereby catalyzing the reaction |
D. | Enzyme specificity is induced by enzyme-substrate binding |
Answer» C. Enzyme-substrate binding induces an increase in the reaction entropy, thereby catalyzing the reaction | |
3. |
Which of the following state represents the condition – “Initial mixing of E+S, while [ES] builds up”. |
A. | Enzyme is saturated with substrate |
B. | Pre- Steady state |
C. | Steady – state |
D. | Steady-state kinetics |
Answer» C. Steady – state | |
4. |
What is the unit of catalytic efficiency kcat/Km? |
A. | conc-1 time-1 |
B. | conc time |
C. | conc-2time-2 |
D. | conc time-1 |
Answer» B. conc time | |
5. |
Km is directly proportional to the rate of enzyme binding? |
A. | True |
B. | False |
Answer» C. | |
6. |
Is vmax affected by enzyme concentration? |
A. | True |
B. | False |
Answer» B. False | |
7. |
What is the unit of vmax? |
A. | mmol |
B. | mol/sec |
C. | mol |
D. | mol/hr |
Answer» C. mol | |
8. |
What is the term “Km”? |
A. | Concentration of the enzyme |
B. | Concentration of the catalyst |
C. | Concentration of the product |
D. | Concentration of the substrate |
Answer» E. | |
9. |
Which plot represents the following equation? |
A. | \(\frac{s}{v} = \frac{K_m}{v_{max}} + \frac{s}{v_{max}}\) |
B. | Line-weaver burk plot |
C. | Eadie-Hofstee plot |
D. | Michaelis-menten plot |
E. | Langmuir plot |
Answer» E. Langmuir plot | |