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This section includes 37 Mcqs, each offering curated multiple-choice questions to sharpen your Bioprocess Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Continuous addition of sugars in “Fed-batch” fermentation is done to __________ |
| A. | Produce methane |
| B. | Purify enzymes |
| C. | Degrade sewage |
| D. | Obtain antibiotics |
| Answer» C. Degrade sewage | |
| 2. |
Which of the following is not a disadvantage of continuous culture? |
| A. | Long growth periods of subtrates/microbes |
| B. | Maintenance of mixed cultures |
| C. | Requires feed-batch culturing |
| D. | Viscosity of mixture for filamentous organisms |
| Answer» C. Requires feed-batch culturing | |
| 3. |
Which of the following is not an advantage of continuous culture? |
| A. | Can be used for different reactions every day |
| B. | Little risk of infection or strain mutation |
| C. | Long growth periods of subtrates/microbes |
| D. | Eliminating the inherent down time for cleaning and sterilization |
| Answer» D. Eliminating the inherent down time for cleaning and sterilization | |
| 4. |
Mixed cultures can be maintained using chemostat cultures in _________ |
| A. | Fed-Batch culture |
| B. | Semi-Batch culture |
| C. | Batch culture |
| D. | Continuous culture |
| Answer» E. | |
| 5. |
“The growth rate of the cells will be greater than the dilution rate”, Is this statement applicable for decrease in biomass production? |
| A. | True |
| B. | False |
| Answer» C. | |
| 6. |
If one starts with 10,000 (104) cells in a culture that has a generation time of 2 h, how many cells will be in the culture after 4 and 48 h? |
| A. | 4.0×104 cells, 1.7×1011 cells |
| B. | 4.2×104 cells, 1.1×1011 cells |
| C. | 4.6×104 cells, 1.5×1011 cells |
| D. | 4.8×104 cells, 1.3×1011 cells |
| Answer» B. 4.2×104 cells, 1.1×1011 cells | |
| 7. |
In the following equation, what is the unit of Dilution rate”D”? (Given: F = dm3h-1 and V = dm3)D = F/V |
| A. | dm3 |
| B. | h-1 |
| C. | dmh-1 |
| D. | dm3h-1 |
| Answer» C. dmh-1 | |
| 8. |
Which of the following is NOT correct for Continuous fermentation? |
| A. | Setup is not changed from outside once the fermentation has started |
| B. | Nutrients are added many times |
| C. | Closer to the natural environment |
| D. | The small size of fermenters are required |
| Answer» B. Nutrients are added many times | |
| 9. |
The continuous culture or fermentation can be used to produce _______ |
| A. | Biomass |
| B. | Primary metabolites |
| C. | Secondary metabolites |
| D. | Antibodies |
| Answer» C. Secondary metabolites | |
| 10. |
Which carbon source has great application for SCP production? |
| A. | Cellulose |
| B. | Starch |
| C. | Methanol |
| D. | Methane |
| Answer» B. Starch | |
| 11. |
All the constituents of medium are excess in _______ |
| A. | Chemostat |
| B. | Turbidostat |
| C. | Batch culture |
| D. | Fed-Batch culture |
| Answer» B. Turbidostat | |
| 12. |
Which of the following is not the type of continuous brewing? |
| A. | Cascade system |
| B. | Multistage system |
| C. | Tower system |
| D. | Extract brewing |
| Answer» E. | |
| 13. |
In continuous culture, the turnover rate is high. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 14. |
What are the disadvantages of continuous culture? |
| A. | It can rarely be used for other productions |
| B. | The growth rate is higher |
| C. | If contamination occurs, huge volumes of the product may be lost |
| D. | It is very useful for processes involving the production of primary metabolites |
| Answer» D. It is very useful for processes involving the production of primary metabolites | |
| 15. |
A multistage system is shown below. Label the stages 1 and 2. |
| A. | 1-Medium inlet and 2-culture effluent |
| B. | 1-Mixing chamber and 2-culture effluent |
| C. | 1-Medium inlet and 2-Liquid reservoir |
| D. | 1-Culture effluent and 1-Medium Inlet |
| Answer» B. 1-Mixing chamber and 2-culture effluent | |
| 16. |
Which scientist(s) gave a full kinetic description of external and internal feedback system? |
| A. | Monod |
| B. | Pirt |
| C. | Bull |
| D. | Harte and Webb |
| Answer» C. Bull | |
| 17. |
The chemostat and turbidostat are the types of bioreactors that are used in which of the following culture? |
| A. | Batch culture |
| B. | Continuous culture |
| C. | Fed-Batch culture |
| D. | Solid State culture |
| Answer» C. Fed-Batch culture | |
| 18. |
What is the unit of flow rate (F)? |
| A. | dm3 h-1 |
| B. | km3 h-1 |
| C. | ml3 h-2 |
| D. | dm2 h-2 |
| Answer» B. km3 h-1 | |
| 19. |
What is the dilution rate (D)? |
| A. | D = F/V |
| B. | D = V/F |
| C. | D = µF |
| D. | D = µD |
| Answer» B. D = V/F | |
| 20. |
Which Growth phase is usually longer in continuous culture? |
| A. | Log |
| B. | Exponential |
| C. | Stationary |
| D. | Death |
| Answer» C. Stationary | |
| 21. |
During continuous culture/fermentation, nutrients in the fermenter are utilized at a fast rate. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 22. |
The Continuous culture is a/an ______ culture system. |
| A. | Open |
| B. | Closed |
| C. | Isolated |
| D. | Semi-closed |
| Answer» B. Closed | |
| 23. |
MIXED_CULTURES_CAN_BE_MAINTAINED_USING_CHEMOSTAT_CULTURES_IN__________?$ |
| A. | Fed-Batch culture |
| B. | Semi-Batch culture |
| C. | Batch culture |
| D. | Continuous culture |
| Answer» E. | |
| 24. |
Which of the following is not a disadvantage of continuous culture?$ |
| A. | Long growth periods of subtrates/microbes |
| B. | Maintenance of mixed cultures |
| C. | Requires feed-batch culturing |
| D. | Viscosity of mixture for filamentous organisms |
| Answer» C. Requires feed-batch culturing | |
| 25. |
Which_of_the_following_is_not_an_advantage_of_continuous_culture?$ |
| A. | Can be used for different reactions every day |
| B. | Little risk of infection or strain mutation |
| C. | Long growth periods of subtrates/microbes |
| D. | Eliminating the inherent down time for cleaning and sterilization |
| Answer» D. Eliminating the inherent down time for cleaning and sterilization | |
| 26. |
Which of the following is used to grow bacterial cultures continuously? |
| A. | Haemostat |
| B. | Chemostat |
| C. | Bacteria cannot be grown in continuous culture |
| D. | Thermostat |
| Answer» C. Bacteria cannot be grown in continuous culture | |
| 27. |
In chemostat, constant cell concentration is maintained? |
| A. | True |
| B. | False |
| Answer» C. | |
| 28. |
Name the phase which is a period of adaptation of the cells to the new environment. |
| A. | Lag phase |
| B. | Log phase |
| C. | Exponential phase |
| D. | Stationary phase |
| Answer» B. Log phase | |
| 29. |
Continuous addition of sugars in “Fed-batch” fermentation is done to __________$ |
| A. | Produce methane |
| B. | Purify enzymes |
| C. | Degrade sewage |
| D. | Obtain antibiotics |
| Answer» C. Degrade sewage | |
| 30. |
Which is the correct meaning of the internal feedback from the following? |
| A. | Effluent stream is more concentrated than in the vessel |
| B. | Effluent stream is less concentrated than in the vessel |
| C. | Effluent stream is equally concentrated with the vessel |
| D. | Effluent stream is not concentrated |
| Answer» C. Effluent stream is equally concentrated with the vessel | |
| 31. |
The yield factor is proportional to the dilution rate? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 32. |
What do you mean by wall growth in bioreactors? |
| A. | Growth of cells in the wall |
| B. | Consumption of paints by the cells from the coated walls |
| C. | Biomass concentration is increased |
| D. | Immobilized cells consumes substrate within reactors |
| Answer» E. | |
| 33. |
An alternative of chemostat is turbidostat? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 34. |
“The growth rate of the cells will be greater than the dilution rate”, Is this statement applicable for decrease in biomass production?$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 35. |
If one starts with 10,000 (104 ) cells in a culture that has a generation time of 2 h, how many cells will be in the culture after 4 and 48 h? |
| A. | 4.0√ó10<sup>4</sup> cells, 1.7√ó10<sup>11</sup> cells |
| B. | 4.2√ó10<sup>4</sup> cells, 1.1√ó10<sup>11</sup> cells |
| C. | 4.6√ó10<sup>4</sup> cells, 1.5√ó10<sup>11</sup> cells |
| D. | 4.8√ó10<sup>4</sup> cells, 1.3√ó10<sup>11</sup> cells |
| Answer» B. 4.2‚Äö√†√∂‚àö‚â•10<sup>4</sup> cells, 1.1‚Äö√†√∂‚àö‚â•10<sup>11</sup> cells | |
| 36. |
under continuous culture which of the following condition is applicable? |
| A. | μ<sub>max</sub> < D |
| B. | μ<sub>max</sub> > D |
| C. | μ<sub>max</sub> = D |
| D. | μ<sub>max</sub>=0 |
| Answer» C. ‚âà√≠¬¨‚à´<sub>max</sub> = D | |
| 37. |
In the following equation, what is the unit of Dilution rate”D”? (Given: F = dm3h-1 and V = dm3) |
| A. | |
| B. | dm<sup>3</sup> |
| C. | h<sup>-1</sup> |
| Answer» C. h<sup>-1</sup> | |