MCQOPTIONS
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MCQOPTIONS
This section includes 74 Mcqs, each offering curated multiple-choice questions to sharpen your Bioprocess Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume) |
| A. | V = V0 + kDVi |
| B. | V = V0/Vi |
| C. | V = V0 - kDVi |
| D. | V/V0 = kDVi |
| Answer» B. V = V0/Vi | |
| 2. |
The paper is non-polar in paper chromatography. |
| A. | True |
| B. | False |
| Answer» C. | |
| 3. |
What useful information can be found from a Van Deemter plot? |
| A. | Optimum column length |
| B. | Optimum column temperature |
| C. | Optimum mobile phase flow rate |
| D. | The selectivity factor |
| Answer» B. Optimum column temperature | |
| 4. |
Theoretical plates are used to ___________ |
| A. | Determine the thickness of the mobile phase |
| B. | Determine the thickness of the stationary phase |
| C. | Estimate the efficiency of the column |
| D. | Measure the distribution of the analyte between mobile and stationary phases |
| Answer» D. Measure the distribution of the analyte between mobile and stationary phases | |
| 5. |
T (0) found to be 2.60 minutes and the sample elutes at 5.70 minutes. Calculate the value of k’ and find the correct answer. |
| A. | 1 |
| B. | 1.19 |
| C. | 1.50 |
| D. | 2.00 |
| Answer» C. 1.50 | |
| 6. |
What does the retention factor, k’, describe? |
| A. | The velocity from the stationary phase |
| B. | The velocity of the mobile phase |
| C. | The distribution of an analyte between the stationary and the mobile phase |
| D. | The migration rate of an analyte through a column |
| Answer» E. | |
| 7. |
Which type of chromatography depends on the principle of size of particles? |
| A. | Affinity chromatography |
| B. | Gel- filtration chromatography |
| C. | Ion- exchange chromatography |
| D. | Multimodal chromatography |
| Answer» C. Ion- exchange chromatography | |
| 8. |
The Rf value is the ratio of distance travelled by solvent to the distance travelled by the solute. |
| A. | True |
| B. | False |
| Answer» C. | |
| 9. |
Thin Layer Chromatography is similar to Paper Chromatography. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 10. |
Chromatography is used to separate ____________ |
| A. | Complex mixture compounds |
| B. | Simple mixtures |
| C. | Viscous mixtures |
| D. | Halogens |
| Answer» B. Simple mixtures | |
| 11. |
The HPLC uses the application of ___________ |
| A. | High temperature |
| B. | Low temperature |
| C. | High pressure |
| D. | Low pressure |
| Answer» D. Low pressure | |
| 12. |
Which of the following dye is widely used in dye-ligand chromatography? |
| A. | Methyl blue |
| B. | Cibracron blue |
| C. | Methyl orange |
| D. | Malachite green |
| Answer» C. Methyl orange | |
| 13. |
Immunoaffinity chromatography is used for the purification of ____________ |
| A. | Lipoproteins |
| B. | Interferons |
| C. | Antibodies |
| D. | Carbohydrates |
| Answer» D. Carbohydrates | |
| 14. |
Which of the following is used to separate molecules based on affinity? |
| A. | Column chromatography |
| B. | Ion exchange chromatography |
| C. | Thin layer chromatography |
| D. | Affinity chromatography |
| Answer» E. | |
| 15. |
Which of the following stationary phase is not used in gel filtration chromatography? |
| A. | Sephadex |
| B. | Sephacryl |
| C. | Bio-Gel |
| D. | Resin beads |
| Answer» E. | |
| 16. |
Which of the following is not a gel filtration chromatography? |
| A. | Molecular sieve |
| B. | Gel permeation |
| C. | Size exclusion |
| D. | Gel residue |
| Answer» E. | |
| 17. |
The charged molecules can be separated by __________ |
| A. | Column chromatography |
| B. | Ion exchange chromatography |
| C. | Thin layer chromatography |
| D. | Affinity chromatography |
| Answer» C. Thin layer chromatography | |
| 18. |
Which of the following is used to pack columns in adsorption chromatography? |
| A. | Carbon |
| B. | Silica gel |
| C. | Potassium hydroxide |
| D. | Aluminium oxide |
| Answer» D. Aluminium oxide | |
| 19. |
Who employed the term ‘Chromatography’? |
| A. | Tsvet |
| B. | Archer |
| C. | Richard |
| D. | Erika |
| Answer» B. Archer | |
| 20. |
Which of the following is used in the precipitation of dextrans? |
| A. | Methanol |
| B. | PEG |
| C. | Ammonium sulphate |
| D. | Sodium sulphate |
| Answer» B. PEG | |
| 21. |
Which of the following is not a stage of product recovery? |
| A. | Removal of solids |
| B. | Isolation of organism |
| C. | Purification and concentration |
| D. | Cell disruption |
| Answer» C. Purification and concentration | |
| 22. |
Which of the following is not a criterion for the choice of the recovery process? |
| A. | Location of the product |
| B. | Price of the product |
| C. | Use of the product |
| D. | Source of organism |
| Answer» E. | |
| 23. |
Which of the following types of chromatography involves the process, where the mobile phase moves through the stationary phase by the influence of gravity or capillary action? |
| A. | Column Chromatography |
| B. | High Pressure Liquid Chromatography |
| C. | Gas Chromatography |
| D. | Planar Chromatography |
| Answer» E. | |
| 24. |
In which of the following type of paper, chromatography does the mobile phase move horizontally over a circular sheet of paper? |
| A. | Ascending paper chromatography |
| B. | Descending paper chromatography |
| C. | Radial paper chromatography |
| D. | Ascending – descending chromatography |
| Answer» D. Ascending – descending chromatography | |
| 25. |
In Thin layer chromatography, the stationary phase is made of _________ and the mobile phase is made of _________ |
| A. | Solid, liquid |
| B. | Liquid, liquid |
| C. | Liquid, gas |
| D. | Solid, gas |
| Answer» B. Liquid, liquid | |
| 26. |
Chromatography cannot be used to separate delicate products. |
| A. | True |
| B. | False |
| Answer» C. | |
| 27. |
In Column chromatography, the stationary phase is made of _________ and the mobile phase is made of _________ |
| A. | Solid, liquid |
| B. | Liquid, liquid |
| C. | Liquid, gas |
| D. | Solid, gas |
| Answer» B. Liquid, liquid | |
| 28. |
Which of the following cannot be used as an adsorbent in Column adsorption chromatography? |
| A. | Magnesium oxide |
| B. | Silica gel |
| C. | Activated alumina |
| D. | Potassium permanganate |
| Answer» E. | |
| 29. |
Chromatography is a physical method that is used to separate and analyse __________ |
| A. | Simple mixtures |
| B. | Complex mixtures |
| C. | Viscous mixtures |
| D. | Metals |
| Answer» C. Viscous mixtures | |
| 30. |
If the mobile phase is gas, movement of solute is determined by its ____________ |
| A. | Boiling point |
| B. | Melting point |
| C. | Solubility |
| D. | Volatility |
| Answer» E. | |
| 31. |
The relative solubility of solute in both the phases determines the ____________ |
| A. | Rate of movement of solvent |
| B. | Rate of disappearance of solvent |
| C. | Rate of movement of solute |
| D. | Rate of disappearance of solute |
| Answer» D. Rate of disappearance of solute | |
| 32. |
A proper solvent that is passed through the column for elution so that separated components can be collected is called ____________ |
| A. | Adsorbent |
| B. | Buffer solution |
| C. | Mobile phase |
| D. | None of the mentioned |
| Answer» C. Mobile phase | |
| 33. |
The gas-solid chromatography is ______________ chromatography as per basic principle involved. |
| A. | Exclusion |
| B. | Ion-exchange |
| C. | Adsorption |
| D. | Absorption |
| Answer» D. Absorption | |
| 34. |
In exclusion chromatography, solute molecules are separated based on ____________ |
| A. | Molecular geometry and size |
| B. | Molecular composition |
| C. | Molecular phase |
| D. | Molecular formula |
| Answer» B. Molecular composition | |
| 35. |
Liquid chromatography is a technique for separating ____________ |
| A. | Ions that are not dissolved in a solvent |
| B. | Ions that are dissolved in a solvent |
| C. | Ions that are dissolved in a solute |
| D. | All of the mentioned |
| Answer» C. Ions that are dissolved in a solute | |
| 36. |
Which of the following is not a stationary phase? |
| A. | Liquid-liquid chromatography |
| B. | Gas-liquid chromatography |
| C. | Gas-solid chromatography |
| D. | Solid-solid chromatography |
| Answer» E. | |
| 37. |
There is no need of support if packed into a column when the stationary phase is ____________ |
| A. | Gas |
| B. | Liquid |
| C. | Solid |
| D. | None of the mentioned |
| Answer» D. None of the mentioned | |
| 38. |
Chromatography involves two mutually ____________ |
| A. | Immiscible phases |
| B. | Miscible phases |
| C. | Soluble phases |
| D. | None of the mentioned |
| Answer» B. Miscible phases | |
| 39. |
Chromatography is the process for identification, purification and separation of components of a mixture on the basis of ____________ |
| A. | Difference in their boiling point |
| B. | Difference in their melting point |
| C. | Difference in their affinity for mobile and stationary phase |
| D. | Difference in their solubility |
| Answer» D. Difference in their solubility | |
| 40. |
THE_RELATIVE_SOLUBILITY_OF_SOLUTE_IN_BOTH_THE_PHASES_DETERMINES_THE?$ |
| A. | Rate of movement of solvent |
| B. | Rate of disappearance of solvent |
| C. | Rate of movement of solute |
| D. | Rate of disappearance of solute |
| Answer» D. Rate of disappearance of solute | |
| 41. |
CHROMATOGRAPHY_CANNOT_BE_USED_TO_SEPARATE_DELICATE_PRODUCTS.?$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 42. |
T_(0)_FOUND_TO_BE_2.60_MINUTES_AND_THE_SAMPLE_ELUTES_AT_5.70_MINUTES._CALCULATE_THE_VALUE_OF_K‚ÄÖ√Ñ√∂‚ÀÖ√Ë‚ÀÖ¬•_AND_FIND_THE_CORRECT_ANSWER.?$# |
| A. | 1.00 |
| B. | 1.19 |
| C. | 1.50 |
| D. | 2.00 |
| Answer» C. 1.50 | |
| 43. |
In which of the following type of paper, chromatography does the mobile phase move horizontally over a circular sheet of paper?$ |
| A. | Ascending paper chromatography |
| B. | Descending paper chromatography |
| C. | Radial paper chromatography |
| D. | Ascending – descending chromatography |
| Answer» D. Ascending ‚Äö√Ñ√∂‚àö√ë‚àö¬® descending chromatography | |
| 44. |
What useful information can be found from a Van Deemter plot?$ |
| A. | Optimum column length |
| B. | Optimum column temperature |
| C. | Optimum mobile phase flow rate |
| D. | The selectivity factor |
| Answer» B. Optimum column temperature | |
| 45. |
If the mobile phase is gas, movement of solute is determined by its$ |
| A. | Boiling point |
| B. | Melting point |
| C. | Solubility |
| D. | Volatility |
| Answer» E. | |
| 46. |
In_Thin_layer_chromatography,_the_stationary_phase_is_made_of___________and_the_mobile_phase_is_made_of__________$ |
| A. | Solid, liquid |
| B. | Liquid, liquid |
| C. | Liquid, gas |
| D. | Solid, gas |
| Answer» B. Liquid, liquid | |
| 47. |
Theoretical_plates_are_used_to____________$ |
| A. | Determine the thickness of the mobile phase |
| B. | Determine the thickness of the stationary phase |
| C. | Estimate the efficiency of the column |
| D. | Measure the distribution of the analyte between mobile and stationary phases |
| Answer» D. Measure the distribution of the analyte between mobile and stationary phases | |
| 48. |
Which of the following types of chromatography involves the process, where mobile phase moves through the stationary phase by the influence of gravity or capillary action? |
| A. | Column Chromatography |
| B. | High Pressure Liquid Chromatography |
| C. | Gas Chromatography |
| D. | Planar Chromatography |
| Answer» E. | |
| 49. |
In Gas-liquid phase chromatography, the stationary phase is composed of _________ and the mobile phase is made of _________ |
| A. | Solid, liquid |
| B. | Liquid, liquid |
| C. | Liquid, gas |
| D. | Solid, gas |
| Answer» D. Solid, gas | |
| 50. |
Gas chromatography can be performed in which of the following ways? |
| A. | Only in columns |
| B. | Only on plane surfaces |
| C. | Either in columns or on plane surfaces |
| D. | Neither in columns nor on plane surfaces |
| Answer» B. Only on plane surfaces | |