

MCQOPTIONS
This section includes 35 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.
1. |
Which of the following bacteria can grow in acidic pH? |
A. | <i>Vibrio cholerae</i> |
B. | <i>Lactobacilli</i> |
C. | <i>Shigella</i> |
D. | <i>Salmonella</i> |
Answer» C. <i>Shigella</i> | |
2. |
A medium containing crystal violet dye plus sodium deoxycholate will allow |
A. | gram (-)ve intestinal bacteria to grow |
B. | gram (+)ve intestinal bacteria to grow |
C. | aquatic bacteria to grow |
D. | none of these |
Answer» B. gram (+)ve intestinal bacteria to grow | |
3. |
Cell size and cell number can be measured using |
A. | hemocytometer |
B. | coulter counter |
C. | petroff-hausser counting chamber |
D. | none of these |
Answer» C. petroff-hausser counting chamber | |
4. |
Which of the following is used for the proper maintenance and preservation of pure cultures? |
A. | Periodic transfer to fresh media |
B. | Preservation by overlaying cultures with mineral oil |
C. | Preservation by lyophilization |
D. | All of the above |
Answer» E. | |
5. |
Peptone water and nutrient broth are |
A. | selective media |
B. | enriched media |
C. | basal media |
D. | none of these |
Answer» D. none of these | |
6. |
Stuart's transport medium is used for transport of specimen containing |
A. | <i>Neisseria gonorrhoeae</i> |
B. | <i>Salmonella </i> |
C. | <i>Vibrio cholerae</i> |
D. | <i>Shigella</i> |
Answer» B. <i>Salmonella </i> | |
7. |
Plate counts are often reported as |
A. | cfu |
B. | cfu/ml |
C. | Both (a) and (b) |
D. | thousands/ml |
Answer» C. Both (a) and (b) | |
8. |
The medium which allows the growth of more than one microorganisms of interest but with morphologically distinguishable colonies is known as |
A. | selective medium |
B. | enrichment medium |
C. | differential medium |
D. | none of these |
Answer» D. none of these | |
9. |
Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectively isolate |
A. | coliform bacteria |
B. | gram positive bacteria |
C. | yeasts |
D. | acid fast organisms |
Answer» D. acid fast organisms | |
10. |
Which of the following hint(s) suggest/s that a given specimen is likely to contain anaerobic bacteria? |
A. | Gas in specimen |
B. | Foul odour |
C. | Presence of sulphur granules |
D. | All of these |
Answer» E. | |
11. |
Viable plate count can be obtained using |
A. | Spread plate method |
B. | Pour plate method |
C. | both (a) and (b) |
D. | Hemocytometer |
Answer» D. Hemocytometer | |
12. |
In the pour plate method, the mixed culture is diluted directly in tubes of |
A. | liquid agar medium |
B. | sterile liquid usually water |
C. | both (a) and (b) |
D. | none of these |
Answer» B. sterile liquid usually water | |
13. |
When a substance is added to a solid medium which inhibits the growth of unwanted bacteria but permits the growth of wanted bacteria, it is known as |
A. | differential medium |
B. | enriched medium |
C. | enrichment medium |
D. | selective medium |
Answer» E. | |
14. |
Important example(s) of enriched medium/media is/ are |
A. | Loeffler's serum slope |
B. | Bordet-gengou medium |
C. | Blood agar |
D. | All of these |
Answer» E. | |
15. |
Which of the following bacteria can grow in alkaline pH? |
A. | <i>Shigella</i> |
B. | <i>Lactobacilli</i> |
C. | <i>Salmonella</i> |
D. | <i>Vibrio cholerae</i> |
Answer» E. | |
16. |
All bacteria that inhabit the human body are |
A. | heterotrophs |
B. | chemolithotrophs |
C. | autotrophs |
D. | phototroph |
Answer» B. chemolithotrophs | |
17. |
When a substance is added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria, it is known as |
A. | differential medium |
B. | enriched medium |
C. | enrichment medium |
D. | selective medium |
Answer» D. selective medium | |
18. |
Cell counting can be carried out by |
A. | direct microscopic count using petroffhansser counting chamber |
B. | plate counting |
C. | membrane filter count |
D. | all of the above |
Answer» E. | |
19. |
Which of the following methods is most likely to be quantitative? |
A. | Dilution and plating |
B. | Gram staining |
C. | Wet mount |
D. | None of these |
Answer» B. Gram staining | |
20. |
The concentration of agar to obtain semi solid media is |
A. | 1.5-20% |
B. | 0.5% or less |
C. | >10% |
D. | >10%but<20% |
Answer» C. >10% | |
21. |
Which of the following problem makes it impossible to satisfy all of Koch's postulates? |
A. | Microorganism causes serious symptoms in humans |
B. | Microorganism cannot be isolated in pure culture |
C. | Species of microorganism is in question |
D. | Genes from the microorganism cannot be amplified by PCR |
Answer» C. Species of microorganism is in question | |
22. |
Mac-Conkey medium is an example of |
A. | transport medium |
B. | enrichment medium |
C. | differential medium |
D. | all of these |
Answer» D. all of these | |
23. |
Pseudomonas aeruginosa forms a blue water soluble pigment called |
A. | pyocyanin |
B. | chlororaphin |
C. | pyoverdin |
D. | -carotene |
Answer» B. chlororaphin | |
24. |
To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of |
A. | 8.5 |
B. | 8.90 |
C. | 6.79 |
D. | 3.5 |
Answer» B. 8.90 | |
25. |
For the selection of endospore-forming bacteria, a mixed culture can be heated at |
A. | 80 C for 10 minutes |
B. | 70 C for 10 minutes |
C. | 60 C for 10 minutes |
D. | 90 C for 10 minutes |
Answer» B. 70 C for 10 minutes | |
26. |
A disease producing species occurring in a mixed culture can often be selected on the basis of its |
A. | pathogenic properties |
B. | special carbon source |
C. | special nitrogen source |
D. | none of these |
Answer» B. special carbon source | |
27. |
Agar is used for solidifying culture media because |
A. | it does not affect by the growth of bacteria |
B. | it does not add to the nutritive properties of the medium |
C. | the melting and solidifying points of agar solution are not the same |
D. | all of the above |
Answer» E. | |
28. |
For the selection of lactobacilli present in cheddar cheese, the pH of the medium is maintained at |
A. | 6.35 |
B. | 5.35 |
C. | 4.35 |
D. | 5.75 |
Answer» C. 4.35 | |
29. |
In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at |
A. | -60 to -78 C |
B. | -20 to -30 C |
C. | -30 to -48 C |
D. | -48 to -58 C |
Answer» B. -20 to -30 C | |
30. |
The solidifying agent commonly used in preparation of media is/are |
A. | agar |
B. | silica gel |
C. | both (a) and (b) |
D. | none of these |
Answer» D. none of these | |
31. |
Treponema species from human oral cavity can be selected by using |
A. | pH of the medium |
B. | incubation temperature |
C. | cell size and motility |
D. | all of these |
Answer» D. all of these | |
32. |
Which of the following techniques may be performed quantitatively to determine the number of bacteria of a particular type? |
A. | Pour plate |
B. | Spread plate |
C. | Both (a) and (b) |
D. | Streak plate technique |
Answer» D. Streak plate technique | |
33. |
Which of the following chemotherapeutic agents may affect the growth of Campylobacter jejuni? |
A. | Vacomycin |
B. | Polymyxin |
C. | Trimethoprim |
D. | None of these |
Answer» E. | |
34. |
Which of the following bacteria dies quickly after drying? |
A. | <i>Treponema pallidium</i> |
B. | <i>Mycobacterium tuberculosis</i> |
C. | <i>Staphylococcus aureus</i> |
D. | <i>Pseudomonas aeruginosa</i> |
Answer» B. <i>Mycobacterium tuberculosis</i> | |
35. |
Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited. |
A. | bile salts |
B. | low concentration of various dyes |
C. | sugars |
D. | low levels of nitrogen |
Answer» B. low concentration of various dyes | |