35 + Mcqs in Cell Cultures and Characteristics Page 1 McqOptions

1.	Which of the following bacteria can grow in acidic pH?
A.	<i>Vibrio cholerae</i>
B.	<i>Lactobacilli</i>
C.	<i>Shigella</i>
D.	<i>Salmonella</i>
Answer» C. <i>Shigella</i>

Discussion

2.	A medium containing crystal violet dye plus sodium deoxycholate will allow
A.	gram (-)ve intestinal bacteria to grow
B.	gram (+)ve intestinal bacteria to grow
C.	aquatic bacteria to grow
D.	none of these
Answer» B. gram (+)ve intestinal bacteria to grow

Discussion

3.	Cell size and cell number can be measured using
A.	hemocytometer
B.	coulter counter
C.	petroff-hausser counting chamber
D.	none of these
Answer» C. petroff-hausser counting chamber

Discussion

4.	Which of the following is used for the proper maintenance and preservation of pure cultures?
A.	Periodic transfer to fresh media
B.	Preservation by overlaying cultures with mineral oil
C.	Preservation by lyophilization
D.	All of the above
Answer» E.

Discussion

5.	Peptone water and nutrient broth are
A.	selective media
B.	enriched media
C.	basal media
D.	none of these
Answer» D. none of these

Discussion

6.	Stuart's transport medium is used for transport of specimen containing
A.	<i>Neisseria gonorrhoeae</i>
B.	<i>Salmonella </i>
C.	<i>Vibrio cholerae</i>
D.	<i>Shigella</i>
Answer» B. <i>Salmonella </i>

Discussion

7.	Plate counts are often reported as
A.	cfu
B.	cfu/ml
C.	Both (a) and (b)
D.	thousands/ml
Answer» C. Both (a) and (b)

Discussion

8.	The medium which allows the growth of more than one microorganisms of interest but with morphologically distinguishable colonies is known as
A.	selective medium
B.	enrichment medium
C.	differential medium
D.	none of these
Answer» D. none of these

Discussion

9.	Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectively isolate
A.	coliform bacteria
B.	gram positive bacteria
C.	yeasts
D.	acid fast organisms
Answer» D. acid fast organisms

Discussion

10.	Which of the following hint(s) suggest/s that a given specimen is likely to contain anaerobic bacteria?
A.	Gas in specimen
B.	Foul odour
C.	Presence of sulphur granules
D.	All of these
Answer» E.

Discussion

11.	Viable plate count can be obtained using
A.	Spread plate method
B.	Pour plate method
C.	both (a) and (b)
D.	Hemocytometer
Answer» D. Hemocytometer

Discussion

12.	In the pour plate method, the mixed culture is diluted directly in tubes of
A.	liquid agar medium
B.	sterile liquid usually water
C.	both (a) and (b)
D.	none of these
Answer» B. sterile liquid usually water

Discussion

13.	When a substance is added to a solid medium which inhibits the growth of unwanted bacteria but permits the growth of wanted bacteria, it is known as
A.	differential medium
B.	enriched medium
C.	enrichment medium
D.	selective medium
Answer» E.

Discussion

14.	Important example(s) of enriched medium/media is/ are
A.	Loeffler's serum slope
B.	Bordet-gengou medium
C.	Blood agar
D.	All of these
Answer» E.

Discussion

15.	Which of the following bacteria can grow in alkaline pH?
A.	<i>Shigella</i>
B.	<i>Lactobacilli</i>
C.	<i>Salmonella</i>
D.	<i>Vibrio cholerae</i>
Answer» E.

Discussion

16.	All bacteria that inhabit the human body are
A.	heterotrophs
B.	chemolithotrophs
C.	autotrophs
D.	phototroph
Answer» B. chemolithotrophs

Discussion

17.	When a substance is added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria, it is known as
A.	differential medium
B.	enriched medium
C.	enrichment medium
D.	selective medium
Answer» D. selective medium

Discussion

18.	Cell counting can be carried out by
A.	direct microscopic count using petroffhansser counting chamber
B.	plate counting
C.	membrane filter count
D.	all of the above
Answer» E.

Discussion

19.	Which of the following methods is most likely to be quantitative?
A.	Dilution and plating
B.	Gram staining
C.	Wet mount
D.	None of these
Answer» B. Gram staining

Discussion

20.	The concentration of agar to obtain semi solid media is
A.	1.5-20%
B.	0.5% or less
C.	>10%
D.	>10%but<20%
Answer» C. >10%

Discussion

21.	Which of the following problem makes it impossible to satisfy all of Koch's postulates?
A.	Microorganism causes serious symptoms in humans
B.	Microorganism cannot be isolated in pure culture
C.	Species of microorganism is in question
D.	Genes from the microorganism cannot be amplified by PCR
Answer» C. Species of microorganism is in question

Discussion

22.	Mac-Conkey medium is an example of
A.	transport medium
B.	enrichment medium
C.	differential medium
D.	all of these
Answer» D. all of these

Discussion

23.	Pseudomonas aeruginosa forms a blue water soluble pigment called
A.	pyocyanin
B.	chlororaphin
C.	pyoverdin
D.	-carotene
Answer» B. chlororaphin

Discussion

24.	To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of
A.	8.5
B.	8.90
C.	6.79
D.	3.5
Answer» B. 8.90

Discussion

25.	For the selection of endospore-forming bacteria, a mixed culture can be heated at
A.	80 C for 10 minutes
B.	70 C for 10 minutes
C.	60 C for 10 minutes
D.	90 C for 10 minutes
Answer» B. 70 C for 10 minutes

Discussion

26.	A disease producing species occurring in a mixed culture can often be selected on the basis of its
A.	pathogenic properties
B.	special carbon source
C.	special nitrogen source
D.	none of these
Answer» B. special carbon source

Discussion

27.	Agar is used for solidifying culture media because
A.	it does not affect by the growth of bacteria
B.	it does not add to the nutritive properties of the medium
C.	the melting and solidifying points of agar solution are not the same
D.	all of the above
Answer» E.

Discussion

28.	For the selection of lactobacilli present in cheddar cheese, the pH of the medium is maintained at
A.	6.35
B.	5.35
C.	4.35
D.	5.75
Answer» C. 4.35

Discussion

29.	In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at
A.	-60 to -78 C
B.	-20 to -30 C
C.	-30 to -48 C
D.	-48 to -58 C
Answer» B. -20 to -30 C

Discussion

30.	The solidifying agent commonly used in preparation of media is/are
A.	agar
B.	silica gel
C.	both (a) and (b)
D.	none of these
Answer» D. none of these

Discussion

31.	Treponema species from human oral cavity can be selected by using
A.	pH of the medium
B.	incubation temperature
C.	cell size and motility
D.	all of these
Answer» D. all of these

Discussion

32.	Which of the following techniques may be performed quantitatively to determine the number of bacteria of a particular type?
A.	Pour plate
B.	Spread plate
C.	Both (a) and (b)
D.	Streak plate technique
Answer» D. Streak plate technique

Discussion

33.	Which of the following chemotherapeutic agents may affect the growth of Campylobacter jejuni?
A.	Vacomycin
B.	Polymyxin
C.	Trimethoprim
D.	None of these
Answer» E.

Discussion

34.	Which of the following bacteria dies quickly after drying?
A.	<i>Treponema pallidium</i>
B.	<i>Mycobacterium tuberculosis</i>
C.	<i>Staphylococcus aureus</i>
D.	<i>Pseudomonas aeruginosa</i>
Answer» B. <i>Mycobacterium tuberculosis</i>

Discussion

35.	Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.
A.	bile salts
B.	low concentration of various dyes
C.	sugars
D.	low levels of nitrogen
Answer» B. low concentration of various dyes

Discussion

Explore topic-wise MCQs in Microbiology.

Which of the following bacteria can grow in acidic pH?

A medium containing crystal violet dye plus sodium deoxycholate will allow

Cell size and cell number can be measured using

Which of the following is used for the proper maintenance and preservation of pure cultures?

Peptone water and nutrient broth are

Stuart's transport medium is used for transport of specimen containing

Plate counts are often reported as

The medium which allows the growth of more than one microorganisms of interest but with morphologically distinguishable colonies is known as

Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectively isolate

Which of the following hint(s) suggest/s that a given specimen is likely to contain anaerobic bacteria?

Viable plate count can be obtained using

In the pour plate method, the mixed culture is diluted directly in tubes of

When a substance is added to a solid medium which inhibits the growth of unwanted bacteria but permits the growth of wanted bacteria, it is known as

Important example(s) of enriched medium/media is/ are

Which of the following bacteria can grow in alkaline pH?

All bacteria that inhabit the human body are

When a substance is added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria, it is known as

Cell counting can be carried out by

Which of the following methods is most likely to be quantitative?

The concentration of agar to obtain semi solid media is

Which of the following problem makes it impossible to satisfy all of Koch's postulates?

Mac-Conkey medium is an example of

Pseudomonas aeruginosa forms a blue water soluble pigment called

To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of

For the selection of endospore-forming bacteria, a mixed culture can be heated at

A disease producing species occurring in a mixed culture can often be selected on the basis of its

Agar is used for solidifying culture media because

For the selection of lactobacilli present in cheddar cheese, the pH of the medium is maintained at

In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

The solidifying agent commonly used in preparation of media is/are

Treponema species from human oral cavity can be selected by using

Which of the following techniques may be performed quantitatively to determine the number of bacteria of a particular type?

Which of the following chemotherapeutic agents may affect the growth of Campylobacter jejuni?

Which of the following bacteria dies quickly after drying?

Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.