Which of the following gene have been introduced into the transgenic fish?

Human or rat gene for growth hormone

Chicken gene for delta crystalline protein

E. coli gene for β-galactosidase

Yeast episomal plasmids (YEp) were constructed using

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

12μm yeast plasmid and E coli vector pMB 9

12μm yeast plasmid and E coli vector pBR 322

YAC vectors can be used to obtain artificial chromosomes because it carries

autonomously replicating sequence

To prevent the accumulation of lactate

high glucose concentration is required

low glutamine concentration is required

high glutamine concentration is required

low glucose concentration is required

high glucose concentration is required

The success of embryo transplantation depends upon the ability to obtain an increased number of embryos from animals of superior genetic merit, which can be achieved by the use of

endogenous gonadatrophic hormones

exogenous gonadatrophic hormones

endogenous gonadatrophic hormones

FSH (Follicle Stimulating Hormone)

In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture

Multiple ovulation and embryo transfer (MOET)

can increase the rate of progress in dairy cattle

allows progeny testing of males

allows progeny testing of females

When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose

Yeast Replicating plasmids (YRp) carry

autonomously replicating sequence

Recombinant SV40 viruses introduce the foreign DNA into cells

with the RNA mediated transfection

with the DNA mediated transfection

without the DNA mediated transfection

with the RNA mediated transfection

without the RNA mediated transfection

Higher dissolved oxygen concentration in the culture media are toxic and lead to

metabolism of nutrients in culture media at a rate greater than that required for consumption

cohesive initial site of phage λ

derivative of permissive CV-1 monkey cell line

cohesive initial site of phage λ

derivative of non-permissive CV-1 monkey cell line

Which of the following is correct when a character shows continuous variation assuming a normal distribution?

It is not controlled genetically

It is governed by many genes with small cumulative effect

It is not controlled genetically

Many of the variation is due to fluctuation of climate

WHICH_OF_THE_FOLLOWING_IS_NOT_THE_MAJOR_FUNCTION_OF_THE_SERUM??$

Promotion of tuber and bulb formation

Which of the following is the characteristics of a normal cell?

Dependent on external growth factor

Sub culturing of primary culture

137 + Mcqs in Animal Cell Culture in Life Sciences Page 3 McqOptions

101.	Which of the following gene have been introduced into the transgenic fish?
A.	Human or rat gene for growth hormone
B.	Chicken gene for delta crystalline protein
C.	E. coli gene for β-galactosidase
D.	All of the above
Answer» E.

Discussion

102.	Yeast episomal plasmids (YEp) were constructed using
A.	2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
B.	2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
C.	12μm yeast plasmid and E coli vector pMB 9
D.	12μm yeast plasmid and E coli vector pBR 322
Answer» B. 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

Discussion

103.	Maximum insert size in case of adenovirus vectors is
A.	6-8 kb
B.	4-6 kb
C.	10-12 kb
D.	20 kb
Answer» B. 4-6 kb

Discussion

104.	YAC vectors can be used to obtain artificial chromosomes because it carries
A.	centromere
B.	telomere
C.	autonomously replicating sequence
D.	all of these
Answer» E.

Discussion

105.	Which of the following(s) is/are the most commonly used markers in yeast?
A.	His 3
B.	Ura 3
C.	Trp 1
D.	All of these
Answer» E.

Discussion

106.	Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is
A.	150-300
B.	280-360
C.	300-325
D.	360-400
Answer» C. 300-325

Discussion

107.	Moloney-Mu-leukemia virus is an example of
A.	Vaccinia virus
B.	Baculo virus
C.	Retro virus
D.	none of these
Answer» D. none of these

Discussion

108.	Which of the following is the process of choosing parent organisms for the characteristics that is wanted in their offspring?
A.	Active selection
B.	Reproductive selection
C.	Selective breeding
D.	Breeding selection
Answer» D. Breeding selection

Discussion

109.	To prevent the accumulation of lactate
A.	low glutamine concentration is required
B.	high glutamine concentration is required
C.	low glucose concentration is required
D.	high glucose concentration is required
Answer» D. high glucose concentration is required

Discussion

110.	The success of embryo transplantation depends upon the ability to obtain an increased number of embryos from animals of superior genetic merit, which can be achieved by the use of
A.	exogenous gonadatrophic hormones
B.	endogenous gonadatrophic hormones
C.	both (a) and (b)
D.	FSH (Follicle Stimulating Hormone)
Answer» B. endogenous gonadatrophic hormones

Discussion

111.	In animal cell culture, particularly mammalian cell culture, transformation means
A.	uptake of new genetic material
B.	phenotypic modifications of cells in culture
C.	both (a) and (b)
D.	release of genetic information
Answer» C. both (a) and (b)

Discussion

112.	Disaggregating of cells can be achieved by
A.	physical disruption
B.	enzymatic digestion
C.	treating with chelating agents
D.	all of the above
Answer» E.

Discussion

113.	Exchange of segments between non homologous chromosomes is best known as
A.	translation
B.	translocation
C.	recombination
D.	crossing over
Answer» C. recombination

Discussion

114.	Multiple ovulation and embryo transfer (MOET)
A.	can increase the rate of progress in dairy cattle
B.	allows progeny testing of males
C.	allows progeny testing of females
D.	all of the above
Answer» E.

Discussion

115.	SV 40 vectors, in which late region replacements are done, require
A.	COS cell line
B.	SV 40 helper virus
C.	pBR 322 vector
D.	none of these
Answer» C. pBR 322 vector

Discussion

116.	When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of
A.	incomplete glutamine oxidation
B.	increase in specific lactate production from glucose
C.	both (a) and (b)
D.	accumulation of ammonia
Answer» D. accumulation of ammonia

Discussion

117.	Yeast Replicating plasmids (YRp) carry
A.	centromere
B.	telomere
C.	autonomously replicating sequence
D.	both (a) and (c)
Answer» D. both (a) and (c)

Discussion

118.	Which of the following is the technique used for the embryo culture?
A.	Organ cultures on plasma clots
B.	Organ cultures on agar
C.	Whole embryo cultures
D.	All of these
Answer» E.

Discussion

119.	Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of
A.	Vaccinia virus
B.	Baculo virus
C.	Papilloma virus
D.	Retrovirus
Answer» C. Papilloma virus

Discussion

120.	Recombinant SV40 viruses introduce the foreign DNA into cells
A.	with the DNA mediated transfection
B.	without the DNA mediated transfection
C.	with the RNA mediated transfection
D.	without the RNA mediated transfection
Answer» C. with the RNA mediated transfection

Discussion

121.	Higher dissolved oxygen concentration in the culture media are toxic and lead to
A.	DNA degradation
B.	lipid peroxidation
C.	metabolism of nutrients in culture media at a rate greater than that required for consumption
D.	all of the above
Answer» E.

Discussion

122.	Cos cell line is
A.	cohesive end site of phage λ
B.	derivative of permissive CV-1 monkey cell line
C.	cohesive initial site of phage λ
D.	derivative of non-permissive CV-1 monkey cell line
Answer» C. cohesive initial site of phage λ

Discussion

123.	Autonomously replicating sequence is not present in
A.	YIp
B.	YRp
C.	YCp
D.	YAC
Answer» B. YRp

Discussion

124.	Which of the following is correct when a character shows continuous variation assuming a normal distribution?
A.	It is governed by many genes with small cumulative effect
B.	It is not controlled genetically
C.	Many of the variation is due to fluctuation of climate
D.	None of the above
Answer» B. It is not controlled genetically

Discussion

125.	How many chromosomes are there in the budding yeast (S. cerevisiae)?
A.	16
B.	17
C.	18
D.	20
Answer» B. 17

Discussion

126.	Raphanobrassica is a classical example of
A.	aneuploidy
B.	nullisomy
C.	allopolyploidy
D.	None of the above
Answer» D. None of the above

Discussion

127.	In a dihybrid cross, the progeny of an Fl individual (hetero2ygous for both characters) will show a phenotypic ratio of
A.	1:1:1:1
B.	1:2:2:1
C.	9:3:3:1
D.	3:1:1:3
Answer» B. 1:2:2:1

Discussion

128.	In an organism, the chromosome number is maintained constant because of
A.	independent assortment
B.	crossing over
C.	DNA duplication
D.	synapsis
Answer» D. synapsis

Discussion

129.	WHICH_OF_THE_FOLLOWING_IS_NOT_THE_MAJOR_FUNCTION_OF_THE_SERUM??$
A.	Promotion of tuber and bulb formation
B.	Stimulate cell growth
C.	Enhance cell attachment
D.	Provide transport proteins
Answer» B. Stimulate cell growth

Discussion

130.	Which of the following is NOT the part of growth medium for animal culture?
A.	Starch
B.	Serum
C.	Carbon source
D.	Inorganic salts
Answer» B. Serum

Discussion

131.	Name the cell line of the human embryonic lung?
A.	HeLa
B.	WISH
C.	L
D.	MRC-5
Answer» E.

Discussion

132.	Which of the following is the characteristics of a normal cell?
A.	Anchorage independent
B.	Continuous cell lines
C.	Dependent on external growth factor
D.	No contact inhibition
Answer» D. No contact inhibition

Discussion

133.	Name the organism on which first cell line was observed?
A.	E.coli
B.	Sheep
C.	Mouse
D.	Drosophila
Answer» D. Drosophila

Discussion

134.	What is a cell line?
A.	Multilayer culture
B.	Transformed cells
C.	Multiple growth of cells
D.	Sub culturing of primary culture
Answer» E.

Discussion

135.	Name the type of culture which is prepared by inoculating directly from the tissue of an organism to culture media?
A.	Primary cell culture
B.	Secondary cell culture
C.	Cell lines
D.	Transformed cell culture
Answer» B. Secondary cell culture

Discussion

136.	Cells from kidney tissues cannot survive independently, it requires other surface for attachment and survival.
A.	True
B.	False
Answer» B. False

Discussion

137.	The growth of animal cells in vitro in a suitable culture medium is called___________
A.	Gene expression
B.	Transgenesis
C.	Plant tissue culture
D.	Animal cell culture
Answer» E.

Discussion

Explore topic-wise MCQs in Life Sciences.

Which of the following gene have been introduced into the transgenic fish?

Yeast episomal plasmids (YEp) were constructed using

Maximum insert size in case of adenovirus vectors is

YAC vectors can be used to obtain artificial chromosomes because it carries

Which of the following(s) is/are the most commonly used markers in yeast?

Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

Moloney-Mu-leukemia virus is an example of

Which of the following is the process of choosing parent organisms for the characteristics that is wanted in their offspring?

To prevent the accumulation of lactate

The success of embryo transplantation depends upon the ability to obtain an increased number of embryos from animals of superior genetic merit, which can be achieved by the use of

In animal cell culture, particularly mammalian cell culture, transformation means

Disaggregating of cells can be achieved by

Exchange of segments between non homologous chromosomes is best known as

Multiple ovulation and embryo transfer (MOET)

SV 40 vectors, in which late region replacements are done, require

When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

Yeast Replicating plasmids (YRp) carry

Which of the following is the technique used for the embryo culture?

Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

Recombinant SV40 viruses introduce the foreign DNA into cells

Higher dissolved oxygen concentration in the culture media are toxic and lead to

Cos cell line is

Autonomously replicating sequence is not present in

Which of the following is correct when a character shows continuous variation assuming a normal distribution?

How many chromosomes are there in the budding yeast (S. cerevisiae)?

Raphanobrassica is a classical example of

In a dihybrid cross, the progeny of an Fl individual (hetero2ygous for both characters) will show a phenotypic ratio of

In an organism, the chromosome number is maintained constant because of

WHICH_OF_THE_FOLLOWING_IS_NOT_THE_MAJOR_FUNCTION_OF_THE_SERUM??$

Which of the following is NOT the part of growth medium for animal culture?

Name the cell line of the human embryonic lung?

Which of the following is the characteristics of a normal cell?

Name the organism on which first cell line was observed?

What is a cell line?

Name the type of culture which is prepared by inoculating directly from the tissue of an organism to culture media?

Cells from kidney tissues cannot survive independently, it requires other surface for attachment and survival.

The growth of animal cells in vitro in a suitable culture medium is called___________