What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would develop normally

The ELISA would not develop when the substrate was added

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

What would happen if the anti-human Ig-conjugate is not washed free of

1.	What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?
A.	The ELISA would develop normally
B.	The ELISA would not develop when the substrate was added
C.	None of the above
D.	All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
Answer» E.