1.

What is the formula for annealing temperature for polymerase chain reaction primer?

A. Tm = 81.5 + 0.41 (%AT) – (675/N)
B. Tm = 81.5 + 0.41 (%GC) – (675/N)
C. Tm = 81.2 + 0.41 (%GC) – (672/N)
D. Tm = 81.5 + 0.42 (%AT) – (675/N)
Answer» C. Tm = 81.2 + 0.41 (%GC) ‚Äö√Ñ√∂‚àö√ë‚àö¬® (672/N)


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