The standard free energy change (?G' ) for ATP hydrolysis is 30 kJ.mole 1. The in vivo concentrations of ATP, ADP and Pi in E. coli are 7.90, 1.04 and 7.90 mM, respectively. When E. coli cells are cultured at 37 C, the free energy change (?G) for ATP hydrolysis in vivo is ______ kJ. mole 1.

• A. - 48 to - 80
• B. - 88 to - 80
• C. - 48 to - 46
• D. - 55 to - 44