The efficiency of amplification in PCR in later cycles is reduced due

1.	The efficiency of amplification in PCR in later cycles is reduced due to
A.	reduction in substrate concentration
B.	insufficient enzyme and time to synthesize mass quantity of DNA
C.	build up of PCR product which competes with primers for hybrid formation
D.	all of these
Answer» E.

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The efficiency of amplification in PCR in later cycles is reduced due to

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