The activity of lactate dehydrogenase can be measured by monitoring the following reaction: Pyruvate + NADH Lactate + NAD+The molar extinction coefficient of NADH at 340 nm is 6220 M 1.cm 1.NAD+ does not absorb at this wavelength. In an assay, 25 L of a sample of enzyme (containing 5 g protein per mL) was added to a mixture of pyruvate and NADH to give a total volume of 3 mL in a cuvette of 1 cm pathlength. The rate of decrease in absorbance at 340 nm was 0.14 min 1. The specific activity of the enzyme will be _________ mol.min 1.mg 1.

The activity of lactate dehydrogenase can be measured by monitoring th

1.	The activity of lactate dehydrogenase can be measured by monitoring the following reaction: Pyruvate + NADH Lactate + NAD+The molar extinction coefficient of NADH at 340 nm is 6220 M 1.cm 1.NAD+ does not absorb at this wavelength. In an assay, 25 L of a sample of enzyme (containing 5 g protein per mL) was added to a mixture of pyruvate and NADH to give a total volume of 3 mL in a cuvette of 1 cm pathlength. The rate of decrease in absorbance at 340 nm was 0.14 min 1. The specific activity of the enzyme will be _________ mol.min 1.mg 1.
A.	628.5 : 725.58
B.	845.5 : 958.4
C.	525.0 : 555.0
D.	225.5 : 452.8
Answer» D. 225.5 : 452.8

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