Temperature required for various steps of polymerase chain reaction (i), (ii) and (iii) are: (i) Denaturation (ii) Annealing (iii) Extension

• A. (i) \[-94{}^\circ C\], (ii) \[-60{}^\circ C\], (iii) \[-72{}^\circ C\]
• B. (i)\[-40{}^\circ C\], (ii) \[-72{}^\circ C\], (iii) \[-94{}^\circ C\]
• C. (i) \[-72{}^\circ C\] (ii) - 94°C, (iii) \[-40{}^\circ C\]
• D. (i) \[-90{}^\circ C\], (ii) \[-72{}^\circ C\], (iii) \[-40{}^\circ C\]