Which of the following is used to determine the appropriate stringency (Wallace rule)?

Question

TuteeHUB · Accepted Answer

Tm = 69.3  + 0.41 (%)G+C -650/1

TuteeHUB · Answer

Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)

TuteeHUB · Answer

Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)

TuteeHUB · Answer

Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

1.	Which of the following is used to determine the appropriate stringency (Wallace rule)?
A.	Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
B.	Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
C.	Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
D.	Tm = 69.3 + 0.41 (%)G+C -650/1
Answer» D. Tm = 69.3 + 0.41 (%)G+C -650/1

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