1.

Following are the steps of southern blot procedure. (i) Autoradiography (ii) Hybridisation with radioactive nucleic acid (probe). (iii) Blotting (iv) DNA fragments are treated to make them single stranded. (v) Electrophoresis (vi) Clearing of DNA by restriction endonuclease. (vii) Isolation of DNA from sample The correct sequence is:

A. (i), (ii), (iii), (iv), (v), (vi), (vii)
B. (vii), (vi), (v), (iv), (iii), (ii), (i)
C. (i), (ii), (vi), (vii), (iii), (iv), (v)
D. (vii), (vi), (i), (ii), (v), (iii), (iv)
Answer» C. (i), (ii), (vi), (vii), (iii), (iv), (v)


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