1.

Enzyme is immobilised in 8 mm diameter agarose beads at a concentration of 0.018 kg protein m-3 gel. Ten beads are immersed in a well-mixed solution containing 3.2 ×10-3 kg m-3 substrate. The effective diffusivity of substrate in agarose gel is 2.1 × 10-9 m2 s– 1. Kinetics of the enzyme can be approximated as first order with specific rate constant 3.11 × 105 s– 1 per kg protein. Mass transfer effects outside the particles are negligible. Estimate the volume per bead.$

A. 2.65√ó10<sup>-7</sup> m<sup>3</sup>
B. 2.66√ó10<sup>-7</sup> m<sup>3</sup>
C. 2.67√ó10<sup>-7</sup> m<sup>3</sup>
D. 2.68√ó10<sup>-7</sup> m<sup>3</sup>
Answer» E.


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