An enzyme is immobilized on the surface of a non-porous spherical particle of 2 mm diameter. The immobilized enzyme is suspended in a solution having bulk substrate concentration of 10 mM. The enzyme follows first order kinetics with rate constant 10 s 1 and the external mass transfer coefficient is 1 cm.s 1. Assume steady state condition wherein rate of enzyme reaction (m mol.L 1.s 1) at the surface is equal to mass transfer rate (m mol.L 1.s 1). The substrate concentration at the surface of the immobilized particle will be ________ mM.

An enzyme is immobilized on the surface of a non-porous spherical part

1.	An enzyme is immobilized on the surface of a non-porous spherical particle of 2 mm diameter. The immobilized enzyme is suspended in a solution having bulk substrate concentration of 10 mM. The enzyme follows first order kinetics with rate constant 10 s 1 and the external mass transfer coefficient is 1 cm.s 1. Assume steady state condition wherein rate of enzyme reaction (m mol.L 1.s 1) at the surface is equal to mass transfer rate (m mol.L 1.s 1). The substrate concentration at the surface of the immobilized particle will be ________ mM.
A.	8.5 : 8.5
B.	7.5 : 7.5
C.	5.5 : 5.5
D.	2.5 : 2.5
Answer» C. 5.5 : 5.5

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