After completion of Nick translation reaction, 10 l of reaction was spotted on a glass-fiber filter that upon counting resulted into 4.2 104 cpm in reaction. Another 10 l was processed for TCA precipitation to determine redioisotope incorporation. The TCA precipitated sample gave 2.94 104 cpm. What is the percent of [ -32P]-dCTP incorporation into the DNA sample?

After completion of Nick translation reaction, 10 l of reaction was s

1.	After completion of Nick translation reaction, 10 l of reaction was spotted on a glass-fiber filter that upon counting resulted into 4.2 104 cpm in reaction. Another 10 l was processed for TCA precipitation to determine redioisotope incorporation. The TCA precipitated sample gave 2.94 104 cpm. What is the percent of [ -32P]-dCTP incorporation into the DNA sample?
A.	40%
B.	50%
C.	60%
D.	70%
Answer» E.

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