A T-flask is seeded with 105 anchorage-dependent cells. The available area of the T-flask is 25 cm2 and the volume of the medium is 25 ml. Assume that the cells are rectangles of size 5 m 2 m. If the cells grow to monolayer confluence after 50 h, the growth rate in number of cells/(cm2.h) is ____ 105 .

A T-flask is seeded with 105 anchorage-dependent cells. The available

1.	A T-flask is seeded with 105 anchorage-dependent cells. The available area of the T-flask is 25 cm2 and the volume of the medium is 25 ml. Assume that the cells are rectangles of size 5 m 2 m. If the cells grow to monolayer confluence after 50 h, the growth rate in number of cells/(cm2.h) is ____ 105 .
A.	1
B.	2
C.	3
D.	4
Answer» C. 3

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