A DNA fragment of 4500 bp has to be tailed with dT residues by using dTTP and the enzyme terminal transferase . The stock solution of dTTP that is used as a substrate has a concentration of 150 M. Ten l of this stock solution is added to a total volume of 200 l reaction. What will be the concentration of dTTP in the reaction?

A DNA fragment of 4500 bp has to be tailed with dT residues by using d

1.	A DNA fragment of 4500 bp has to be tailed with dT residues by using dTTP and the enzyme terminal transferase . The stock solution of dTTP that is used as a substrate has a concentration of 150 M. Ten l of this stock solution is added to a total volume of 200 l reaction. What will be the concentration of dTTP in the reaction?
A.	7.5 M
B.	75 M
C.	0.75 M
D.	0.075 M
Answer» B. 75 M

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