1.

A bacterial culture having a specific oxygen uptake rate of 5 mmol O2 (g-DCW) 1 hr 1 is being grown aerobically in a fed-batch bioreactor. The maximum value of the volumetric oxygen transfer coefficient is 0.18s 1 for the stirred tank bioreactor and the critical dissolved oxygen concentration is 20% of the saturation concentration (8mg/ml). The maximum density to which the cells can be grown in the fed-batch process without the growth being limited by oxygen transfer, is approximately.

A. 14 g/l
B. 26 g/l
C. 32 g/l
D. 65 g/l
Answer» E.


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