Mention the expression for limit of resolution of microscope.
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Mention the expression for limit of resolution of microscope.
Mention the expression for limit of resolution of microscope.
Minimum separation OR Limit of resolution:
dmin = 1.22λ / 2nsinβ
where λ – wavelength of light, n – refractive index of the medium between the object and the objective lens and 2β – angle subtended by the object at the diameter of the objective lens at the focus of the microscope.